中药材2017，Vol.40Issue(1)：12-17,6.DOI:10.13863/j.issn1001-4454.2017.01.003
金铁锁体外高效再生体系的建立
Efficient Regeneration System of Psammosilene tunicoides in vitro
摘要
Abstract
Objective:An efficient in vitro regeneration system of Psammosilene tunicoides was developed by optimizing the culture condition and selecting the best protocol for plant regeneration.Methods:Effectiveness of plant hormones and concentration were investigated through a single factor pre-experiment,and the sterile seedlings with stem tip,stem with buds and leaves were all used as explants,and MS as the basic culture medium,a L9 (34) orthogonal experiment was designed to explore the effects of different types and concentration of plant hormones and explants on callus induction,adventitious shoot formation and plant regeneration.Results:Stem with buds was the optimal explant for indirect organogenesis with the frequency of callus induction up to 100％,which cultured on MS + 6-BA 1.0 mg/L + NAA 0.1 mg/L + KT 1.0 mg/L,and rate of buds induced and differentiation were up to 100％;the coefficient of differentiation in clump shoot was 16.65 after 30 d and the multiplication coefficient was up to 35.78.However,the stem tip was the best explant for direct organogenesis with the propagation coefficient up to 15.45,which cultured on MS +6-BA 0.1 mg/L + NAA 1.5 mg/L + KT 0.05 mg/L in 30 d later.The regeneration plant with 97.50％ rooting rate was obtained which cultured on 1/2MS +6-BA 0.05 mg/ L + NAA 2.0 mg/L after 45 d,and there were more than 90％ of plantlets survived after transplanting.Conclusion:The current study improves condition of culture and establishes a rapid propagation system of Psammosilene tunicoides.关键词
金铁锁/带芽茎段/正交实验/愈伤组织/繁殖系数Key words
Psammosilene tunicoides W.C.Wu et C.Y.Wu/Stem with buds/Orthogonal test/Callus/Propagation coefficient分类
医药卫生引用本文复制引用
曹磊,钱子刚,黄衡宇,陆莲..金铁锁体外高效再生体系的建立[J].中药材,2017,40(1):12-17,6.基金项目
国家自然科学基金(81260609,31260077) （81260609,31260077）
