中国现代中药2017，Vol.19Issue(4)：504-508,5.DOI:10.13313/j.issn.1673-4890.2017.4.007
UPLC-MS/MS测定不同产地吊石苣苔中苯乙醇苷类成分含量
Determination of Four Phenylethanol Glycosides in Aerial Parts of Lysionotus pauciflorus from Different Habitats by UPLC-MS/MS
摘要
Abstract
Objective:To establish a UPLC-MS/MS method for the simultaneous determination of four phenylethanol glycosides in the aerial parts of Lysionotus panciflorus Maxim.,and analyze and investigate the difference of phenylethanol glycosides ingredients in samples from different habitats.Methods:The determination was performed on the ACQUITY UPLC BEH C18 (100 mm × 2.1 mm,1.8 pμm) with0.1％ formic acid-water(A) and 0.1％ formic acid-acetonitrile(B) in gradient elution as mobile phase.The flow rate was 0.5 ml· min-1.The column temperature was 40 ℃.MS instrument was equipped with ESI-ion source.Gaining The extracted ion chromatograms were obtained susquently.Results:There are differences in the composition and content from different habitats.Conclusion:The developed method for the simultaneous determination of the four phenylethanol glycosides is simple,accurate,and reproducible,has great theoretical significance for looking for high levels of phenylethanol glycosides of plant resources and habitats.关键词
吊石苣苔/苯乙醇苷/UPLC-MS/MSKey words
Lysionotus pauciflorus/phenylethanol glycosides/UPLC-MS/MS引用本文复制引用
张瑜,杨健,詹志来,胡峻,刘勇,袁媛..UPLC-MS/MS测定不同产地吊石苣苔中苯乙醇苷类成分含量[J].中国现代中药,2017,19(4):504-508,5.基金项目
国家自然科学基金项目(81373959) （81373959）
中医药行业科研专项(201407003) （201407003）
北京中医药大学自主选题资助项目(2016-JYB-XS125) （2016-JYB-XS125）
