中国现代中药2017，Vol.19Issue(4)：509-513,5.DOI:10.13313/j.issn.1673-4890.2017.4.008
UPLC-MS同时测定蒙药材蓝刺头中6个成分的含量
Simultaneous Determination of Six Compounds in Echinops latifolius Tausch.Flowers by UPLC-MS
摘要
Abstract
Objective:To develop a specific UPLC-MS method for the determination of chlorogenic acid,1,5-dicaffeoylquinic acid,3,5-dicaffeoylquinic acid,4,5-dicaffeoylquinic acid,apigenin,and apigenin-7-O-glucoside in the flowers of Echinops latifolius Tausch..Methods:By using the UPLC-MS method,the six compounds were separated on an Agilent ZORBAX RRHD Ecilipse Plus-C18 column with a mobile phase consisted of water containing 0.1％ formic acid and methanol with gradient elution.Electrospray ionization source was applied and operated in negative ionization multiple reaction monitoring model(MRM).Results:The linear ranges of chlorogenic acid,3,5-dicaffeoylquinic acid,1,5-dicaffeoylquinic acid,apigenin-7-O-glucoside,4,5-dicaffeoylquinic acid,and apigenin were 1.25-40.00 (r =0.999 0),0.94-30.00(r=0.9998),0.94-30.00(r=0.999 8),0.16-5.00(r=0.9997),0.16-5.00(r=0.9999),0.02-2.50(r=0.999 5) μg· mL-1,respectively.The recoveries of six analytes ranged from 99.80％ to 102.97％,and the relative standard deviations were in the range of 1.23％ to 2.68 ％.Conclusion:The method is sensitive,accuracy and suitable for the determination of main compounds in the flowers of E.latifolius.关键词
蓝刺头/绿原酸类/黄酮类/超高效液相色谱-串联质谱法/含量测定Key words
Echinops latifolius/chlorogenic acids/flavonoids/UPLC-MS/quantification引用本文复制引用
麻剑南,马超美,田志杰,刘卫东..UPLC-MS同时测定蒙药材蓝刺头中6个成分的含量[J].中国现代中药,2017,19(4):509-513,5.基金项目
呼和浩特市重大科技专项(2014150103000169) （2014150103000169）
