中国现代中药2017，Vol.19Issue(4)：518-524,7.DOI:10.13313/j.issn.1673-4890.2017.4.010
左旋龙脑对UVB辐射后小鼠皮肤光损伤的影响
Eeffects of L-borneol on UVB-induced Photo-damages in Skin of Balb/c Mice
摘要
Abstract
Objective:To study effects of L-borneol on interleukin-6 (IL-6)and nuclear factor kappa-B (NF-κB)in UVB irradiation-induced mice.Methods:Eighty of Balb/c mice were randomly divided into four groups.Photo-damaged mouse model was established by UVB irradiation.L-borneol was adopted for wounds once every day for 11 days.The pathological changes of the photo-damaged skin tissue were observed by HE staining.Samples were collected at different time points after UVB irradiation to assess healing time,tissues water content,erythema and edema.The levels of 8-hydroxy-desoxyguanosine (8-OHdG),IL-6 and NF-κB in the epidermis were detected by ELISA.Results:Compared with the normal group,the erythema and edema indexes increased significantly in the UVB model group.The erythema and edema indexes,IL-6 and NF-κB levels in mice with acute UVB exposure significantly reduced after treatment with L-borneol.Further more,HE staining showed that UVB irradiation induced keratinocyte hyperplasia,sunburn cell formation and inflammatory cell infiltration.After treatment with L-borneol,these histological abnormalities were alleviated.In addition,the topical application of L-borneol resulted in a significant decrease in UVB-induced increases of epidermis thickness,especially depressing in UVB mediated generation of 8-OHdG(P ＜ 0.05,P ＜ 0.01).Conclusion:It is considered that L-borneol could alleviate the symptoms of photodamages by UVB irradiation.One principle of the effects could be that L-borneol removed of light products 8-OHdG,than controlled NF-κB regulation on release of IL-6 and lead to fall of IL-6 level in photo-damaged tissue causing role of anti-photo-damage.关键词
左旋龙脑/紫外线/光损伤/IL-6/NF-κBKey words
L-borneol/ultraviolet B/photo-damage/IL-6/NF-κB引用本文复制引用
李小婷,王丹,庞玉新,杨全,范佐旺,马青松,许罗凤..左旋龙脑对UVB辐射后小鼠皮肤光损伤的影响[J].中国现代中药,2017,19(4):518-524,7.基金项目
科技部科研院所技术开发研究专项(2013EG134239) （2013EG134239）
贵州省重大科技专项计划项目(6011-2013) （6011-2013）
