中国现代中药2017，Vol.19Issue(4)：557-559,571,4.DOI:10.13313/j.issn.1673-4890.2017.4.018
地鳖虫抗肿瘤成分提取方法的研究
Study on Extraction of Antitumor Constituents from Eupolyphaga sinensis
摘要
Abstract
Objective:To optimize the extraction of lipophile fraction of Eupolyphaga sinensis.Methods:Through the comparison of Soxhlet extraction and ultrasonic extraction,the extraction conditions for the lipophile fraction were optimized with ultrasonic time,temperature,soaking time of raw material,organic solvent as the factors.Results:In this study,single factor analysis,The extraction rate was 17.23％,and the extraction rate decreased to 13.7％ at 60 min;Ultrasonic temperature at 50 ℃ when the extraction rate of the highest,reaching 15.95％,then the temperature reached 60 ℃ to 14.02％.70 ℃ down to 10％ or less;The highest extraction rate was 18.74％ when soaked for 6 h.While the prolonged soaking time 12h,24h,48h had no effect on the extraction of lipids;Organic solvent anhydrous ether extraction rate of the highest.Conclusion:The optimized condition was as follows:anhydrous ether soak 6 h,ultrasonic temperature 50,ultrasonic time 40 min.关键词
地鳖虫/提取率/超声波Key words
Eupolyphaga sinensis Walker/extraction rate/ultrasonic引用本文复制引用
富尧,张慧明,许龙,吕冬云..地鳖虫抗肿瘤成分提取方法的研究[J].中国现代中药,2017,19(4):557-559,571,4.基金项目
佳木斯大学学研究生创新课题(LZR2014_ 002) （LZR2014_ 002）
佳木斯大学自然科学研究项目(JMSUJCMS2016-064) （JMSUJCMS2016-064）
