中国现代中药2017，Vol.19Issue(5)：617-624,8.DOI:10.13313/j.issn.1673-4890.2017.5.005
滇皖产区铁皮石斛居群SSR分子身份证的构建
Molecular ID for Populations of Dendrobium officinale of Yunnan and Anhui Province Based on SSR Marker
摘要
Abstract
Objective:To establish a molecular ID(mID)for Dendrobium officinale rigin from Yunnan and AnhuiMethods:Genome of Dofficinale was analyzed and SSRs (Simple Sequence Repeats) were isolatedPrimers were designed by Primer Premier 50 software,high polymorphism primers were isolated and genetic diversity analysis was performed,and thenSSR fingerprinting database and mIDs of 11 populations of Dofficinale were constructedResults:A total of 91 653 SSRs were isolated,the average distribution density was 1544 kb75 pairs of SSR primers were designed and screened,32 high polymorphismprimer pairs were selected to generate polymorphic fingerprintsThe results showed that the 32 pairs of primers could generate 117 polymorphic alleles bandsThe average polymorphism information content (PIC)as 062 with a range of 022-08011 populations of Dofficinale could be clustered to 3 branches,which accordance with the geographical distribution of its germplasmFingerprinting database of 11 populations of Dofficinale were established with 4 pairs of primers,and in consequence the molecular ID were established for the 11 populationsConclusion:SSR marker is suitable for construction of DNA fingerprinting database of Dofficinale and the database could provide reference for population identification of Dofficinale.关键词
铁皮石斛/基因组SSR/分子身份证Key words
Dendrobium officinale/genomic SSR/molecular ID引用本文复制引用
董晓曼,袁媛,查良平,彭代银,俞年军,王继永,赵玉洋,蒋超,黄璐琦..滇皖产区铁皮石斛居群SSR分子身份证的构建[J].中国现代中药,2017,19(5):617-624,8.基金项目
中医药行业科研专项(201407003) （201407003）
国家杰出青年科学基金项目(81325023) （81325023）
