中国现代中药2017，Vol.19Issue(5)：652-656,692,6.DOI:10.13313/j.issn.1673-4890.2017.5.012
炮天雄传统炮制过程中微生物的分离与初步鉴定
Identification and Isolation of Microorganisms from Processed Aconitum Carmichnaeli Samples during Its Processing
摘要
Abstract
Objective:To reveal the beneficial microorganisms in Processed Aconitum Carmichnaeli during its processing by traditional fermented methodsMethods:By classical microbial isolation and purification methods,the strains in Processed Aconitum Carmichnaeli samples were isolated and purifiedThe preliminary taxonomy of the strains isolated was carried out by investigating the morphological properties,analyzing and comparing their 16S rDNA or 18S rDNA gene sequences.Results:14 species of bacteria,11 species of yeasts and 2 species of filamentous fungi were isolated and identified in Processed Aconitum Carmichnaeli samplesThe bacteria included Klebsiella spp,Escherichia coli,Arthrobacter spp,Bacillus cereus,Bacillus pumilus,Lysinibacillus fusiformis,Bergeyella spEmpedobacter brevis,Corynebacterium sp,Salinicoccus hispanicus,Bacillus meqaterium partial,Weissella cibaria,Lactobacillus plantarum,Lactococcus IactisThe yeasts included Corynebacterium bovis,Rhodotorula mucilaginosa,Clavispora lusitaniae,Candida tropicalis,Pichia kluyveri,Trichosporon spp,Galactomyces geotrichum,Geotrichum spp,Pichia barkeri,Candida athensensis spp,Geotrichum candidum,and the fungi are Penicillium chrysogenum and Mucor circinelloidesConclusion:Many species of microorganisms exist in Processed Aconitum Carmichnaeli samples during its processing,which help to reconstruct modern fermentation technology of Processed Aconitum Carmichnaeli.关键词
炮天雄/炮制/微生物/分离/鉴定Key words
Processed Aconitum Carmichnaeli/fermentation/microorganism/isolation/identification引用本文复制引用
胡梦,杜杰,贾丹丹,刘英,胡海峰..炮天雄传统炮制过程中微生物的分离与初步鉴定[J].中国现代中药,2017,19(5):652-656,692,6.基金项目
"十二五"重大新药专项(2014ZX09201001-005-001) （2014ZX09201001-005-001）
国家中医药管理局中医药行业专项(201507004) （201507004）
