中医药信息2017，Vol.34Issue(3)：20-23,4.
不同产地意大利牛舌草中芦丁和山奈酚的含量测定及其抗补体活性研究
Content Determination of Rutin and Kaempferol in Anchusa Italica Retz.from Different Regions and Its Anti-complement Activity
摘要
Abstract
Objective:To determine the content of rutin and kaempferol in Anchusa italica Retz.from different regions and its anti-complement activity.Methods:UPLC was used,and the analysis was performed on a ACQUITY UPLC BEH C18(100 mm × 2.1 mm,1.7 μm)with methanol (A)-0.4％ formic acid solution (B) as the mobile phases with gradient elution (0-3 min,5-31％ A;3-10 min,31％-37％ A;10-12 min,37％-40％ A;12-30 min,40％-40％ A).The column temperature was set at 30℃,and the UV detection wavelength was 366 nm.Anti-complement activity of rutin and kaempferol was evaluated by hemolysis test.Results:The contents of rutin and kaempferol in Anchusa italica Retz were in the range of 1.25-3.34 mg/g and 2.86-6.05 mg/g,respectively.Rutin and kaempferol had a good anti-complement aetivity.Conclusion:There is no significant difference in the contents of two components in Anchusa italica Retz from the same region,but has differences in different regions.The two components have a good anti-complement activity.关键词
意大利牛舌草/含量测定/芦丁/山奈酚/抗补体活性Key words
Anchusa italica Retz./Content determination/Rutin/Kaempferol/Anti-complement activity分类
医药卫生引用本文复制引用
何媛媛,韩红园,李二文,代一航,索亚然,林瑞超..不同产地意大利牛舌草中芦丁和山奈酚的含量测定及其抗补体活性研究[J].中医药信息,2017,34(3):20-23,4.基金项目
国家中医药管理局公益性行业科研专项项目(No.201507004) （No.201507004）
