组织工程与重建外科杂志2017，Vol.13Issue(3)：131-135,159,6.DOI:10.3969/j.issn.1673-0364.2017.03.004
细胞-凝胶复合物促进聚己内酯内核与细胞膜片组织整合的可行性研究
The Feasibility of Using Cell-Hydrogel to Integrate PCL Inner Support with Cell-Sheet
徐亚文 1何爱娟 2刘豫 1周广东 2曹谊林2
作者信息
- 1. 200011 上海市 上海交通大学医学院附属第九人民医院整复外科
- 2. 200011 上海市上海市组织工程研究重点实验室
- 折叠
摘要
Abstract
Objective To explore the feasibility of using sandwich model, PCL as an inner support encapsulated by chondrocyte sheet, to create tissue-engineered (TE) cartilage; To explore the feasibility of integrating PCL with chondrocyte sheet by injecting the mixture of Pluronic F-127 hydrogel and chondrocytes. Methods The isolated auricular chondrocytes of passage 1 were seeded in six-well plate at a high density. Chondrocyte sheet formed after 4 weeks in vitro. PCL inner support encapsulated by chondrocyte sheet was set up as non-treated control group (NC group). Sandwich model injected with Pluronic F-127 hydrogel was set up as control group (PC group). Sandwich model injected with the mixture of Pluronic F-127 hydrogel and chondrocytes was set up as the experiment group (Exp group). The above constructs were implanted subcutaneously into the same nude mice (n=8). The samples were harvested 12 weeks after implantation for the evaluation of cartilage formation. Results After in vitro high cell-density culture, chondrocyte sheet formed into cartilaginous tissue. Histologically, sheet showed specific cartilage characters including cartilage lacunae and expressed GAG and collagen Ⅱ. After in vivo culture, sheet from the "sandwich" structure formed more mature cartilaginous tissue with smooth surface and flexible texture. The results of wet weight, thickness and volume from every group were significantly different. However, there was no significant difference for Young's modulus. Histologically, an abundance of cartilage-like tissue was found between PCL inner support and chondrocyte sheet in Exp group, strongly expressing GAG. Although sheet developed into mature cartilage tissue in the NC and PC group, none of cartilaginous tissue was found between the PCL inner support and chondrocyte sheet. Conclusion PCL inner support encapsulated by chondrocyte sheet could construct TE cartilage with high mechanical strength. The approach of injection with the mixture of Pluronic F-127 hydrogel and chondrocytes could realize the cartilaginous integration of PCL inner support and chondrocyte sheet.关键词
组织工程/聚己内酯内核/软骨膜片/温敏凝胶Key words
Tissue engineering/PCL inner support/Cartilage sheet/Thermo-sensitive gel分类
生物科学引用本文复制引用
徐亚文,何爱娟,刘豫,周广东,曹谊林..细胞-凝胶复合物促进聚己内酯内核与细胞膜片组织整合的可行性研究[J].组织工程与重建外科杂志,2017,13(3):131-135,159,6.
