癌变·畸变·突变2017，Vol.29Issue(4)：245-250,6.DOI:10.3969/j.issn.1004-616x.2017.04.001
Wnt蛋白生成抑制剂2对非小细胞 肺癌细胞迁移与侵袭能力的影响
Effects of the inhibitor of Wnt production 2 on cell migration and invasion in non-small cell lung cancer
摘要
Abstract
OBJECTIVE:To investigate the role of the inhibitor of Wnt production 2 (IWP2) on non-small cell lung cancer (NSCLC) cell migration and invasion and to explore mechanisms for the effects.METHODS:H1299 and 95C cell lines were divided into control (cultured with serum-free RPMI-1640 media) and experiment (treated with 10μmol/L IWP2) groups. Cell migration ability was examined with wound healing and with Transwell assay without Matrigel. Cell invasion ability was determined using Transwell assay with Matrigel. In addition,Western blot was performed to analyze the effect of IWP2 onβ-catenin and epithelial-mesenchymal transition(EMT)-related proteins (ZEB1 and Snail). RESULTS:Compared with the control groups,the wound healing results demonstrated that cells cultured with IWP2 had decreased wound closure both in H1299 and 95C (P<0.01). Transwell assay without Matrigel showed that fewer cells migrated to the lower chambers after being treated with IWP2 (P<0.01). The Transwell assay with Matrigel indicated that cell invasion ability was also significantly decreased in the experimental compared with the control groups. Besides,after being treated with IWP2,expression of β-catenin was obviously reduced (41.3% in H1299 and 36.1% in 95C). In addition,IWP2 also decreased the expression of ZEB1 (51.8% in H1299 and 40.9% in 95C) and Snail (43.2% in H1299 and 30.7% in 95C).CONCLUSION:Our study revealed that IWP2 down-regulated EMT via blockingβ-catenin which led to the inhibition of NSCLC cell migration and invasion.关键词
Wnt蛋白生成抑制剂2/非小细胞肺癌/迁移/侵袭/β-cateninKey words
the inhibitor of Wnt production 2/non-small cell lung cancer/migration/invasion/β-catenin分类
医药卫生引用本文复制引用
张静,滕宇,赵晓婷,江妹,岳文涛..Wnt蛋白生成抑制剂2对非小细胞 肺癌细胞迁移与侵袭能力的影响[J].癌变·畸变·突变,2017,29(4):245-250,6.基金项目
国家自然科学基金(81672838,81602531) （81672838,81602531）
