癌变·畸变·突变2017，Vol.29Issue(4)：256-259,265,5.DOI:10.3969/j.issn.1004-616x.2017.04.003
维生素E琥珀酸酯诱导人胃癌细胞发生未折叠蛋白反应的研究
Vitamin E succinate induced unfolded protein response of human gastric cancer SGC-7901 cells
摘要
Abstract
OBJECTIVE:To investigate the effect of vitamin E succinate (VES) on unfolded protein response (UPR) in human gastric cancer SGC-7901 cells.METHODS:SGC-7901 cells were treated with VES at 5,10 and 20μg/mL and with tunicamycin (TM) at 3μg/mL for 24 h or at 20μg/mL for up to 24 h. RNA-dependent protein kinase (PKR)-like ER kinase (PERK) and activating transcription factor 6 (ATF6) were evaluated using Western blotting. X-box-binding protein 1 (XBP1) and activating transcription factor 4 (ATF4) were evaluated using RT-PCR.RESULTS:VES induced the phosphorylation of PERK in a time-dependent manner and reached the top level at 10μg/mL. In addition, VES induced the generation of p50ATF6 in SGC-7901 cells in a dose- and time-dependent manner. Like the positive control,VES treatment at 20μg/mL induced the spliced form of XBP1 from 6h up to 24h. Meanwhile,ATF4 mRNA was also up-regulated in cells exposed to VES in a time-dependent manner and reached the top level at 18 h. CONCLUSION:UPR pathways may be activated by VES in human gastric cancer SGC-7901 cells.关键词
未折叠蛋白反应/维生素E琥珀酸酯/胃癌细胞/内质网应激Key words
unfolded protein response/vitamin E succinate/gastric cancer SGC-7901 cells/endoplasmic reticulum stress分类
医药卫生引用本文复制引用
黄晓莉,吴坤,赵莎莎..维生素E琥珀酸酯诱导人胃癌细胞发生未折叠蛋白反应的研究[J].癌变·畸变·突变,2017,29(4):256-259,265,5.基金项目
国家自然科学基金资助项目(81402663) （81402663）
