曲功霖 1李辰 1邵帅 1王春燕 1齐雪松 1佟鹏 1李宁1
作者信息
- 1. 中国疾病预防控制中心辐射防护与核安全医学所,辐射防护与核应急中国疾病预防控制中心重点实验室,北京 100088
- 折叠
摘要
Abstract
OBJECTIVE:To evaluate the preventive and therapeutic effects of Sijunzi decoction ondamage from ionizing radiation in hematopoietic and immune systems.METHODS:CCK-8 assay kit was used to detect the toxic effect of AHH-1 cells which were treated with different dosages (0,60,120,600,1200μg/mL) ofSijunzi decoction extract for 48 h. In addition,the survival rate of AHH-1 cells which were pretreated with different dosages (0,0.04,0.2,1,25,12060μg/mL) of Sijunzi decoction for 2h and irradiated with 4.0 GyCoγ-rays was investigated. One hundred and sixty BALB/c mice aged 6-8 weeks were divided by random number table method into normal control group,radiation group,and three groups of Sijunzi decoction at dosages of 0.75,2.25 and 6.75 g/kg,respectively. Radiation-damaged mice model were individually-irradiated with 3.5,5.5 Gyγ-rays,respectively. The following a ctivity indexes were investigated:the count and percentage of peripheral lymphocytes,thymus index,survival rate and average survival time in 30 d.RESULTS:Compared to the radiation group,the survival rate of irradiated AHH-1 cells were increased greatly bySijunzi decoction at the dosage of 120μg/mL (P<0.05). At 3d after irradiation,the count and percentage of peripheral lymphocytes were promoted significantly (P<0.05) bySijunzi decoction at the dosage of 2.25 g/kg in mice. The percentage of peripheral lymphocytes was increased significantly (P<0.05) by Sijunzi decoction at the dosage of 6.75 g/kg,also the count of peripheral lymphocytes was increased. At 3 and 7 d after irradiation,the thymus index was increased significantly (P<0.05) by Sijunzi decoction at the dosage of 6.75g/kg. Besides,the survival rate was increased (P<0.05) and the mean survival time was prolonged significantly (P<0.05) by Sijunzi decoction at the dosage of 6.75 g/kg.CONCLUSION:Sijunzi decoction could alleviate the ionizing radiation-induced damage,improve mice immune and hematopoietic function,by means of increasing the count and percentage of peripheral lymphocytes and the thymus index. On the other hand,Sijunzi decoction can also improve the 5.5 Gy-irradiated mice's survival rate. Accordingly,Sijunzi decoction might bed eveloped into a new drug for radiation protection.关键词
四君子汤/电离辐射/淋巴细胞/胸腺/生存率Key words
Sijunzidecoction/ionizing radiation/lymphocytes/thymus/survival rate分类
医药卫生
