癌变·畸变·突变2017，Vol.29Issue(4)：304-308,312,6.DOI:10.3969/j.issn.1004-616x.2017.04.012
LIN28A、 E-cadherin和 vimentin在浸润性乳腺癌中的表达及其临床意义
Expressions of LIN28A, E-cadherin and vimentin in invasive breast cancer
摘要
Abstract
OBJECTIVE:To investigate the expression of RNA-binding proteins LIN28A,epithelial-mesenchymal transition ( EMT) - related p roteins E-cadherin and vimentin in invasive breast cancer and to understand their role ind evelopment of invasive breast cancer.METHODS:Quantitative real-time PCR (qPCR) and immunohistochemical staining were used to detect expressions of LIN28A,E-cadherin and vimentin mRNAs and proteins in invasive breast cancer tissues and the corresponding adjacent tissues. Relationships between mRNA and protein expressions of LIN28A,E-cadherin and vimentin were analyzed. TUNNEL staining was used to detect apoptosis of invasive breast cancer tissues with high LIN28A expressions. The relationship between LIN28A and EMT-related proteins and survival rate of breast cancer patients were statistically analyzed.RESULTS:qPCR results show that the relative expression level of LIN28A mRNA in breast cancer tissues were higher than a djacent tissues, negatively correlated with E-cadherin mRNA,and positively correlated with vimentin mRNA(P<0.05). Immunohitochemistry staining results show that in breast cancer tissues,the expression rates of LIN28A,E-cadherin and vimentin protein were 60%, 55% and 65%,respectively. The expression of LIN28A protein was negatively correlated with E-cadherin protein,positively correlated w ith v imentin protein. LIN28A expression was correlated with tumor size,histological grade and lymph node metastasis. TUNNEL assay show that high expression of LIN28A could d ecrease a poptosis in invasive breast cancer(P<0.05). The survival rate of patients with positively expressed LIN28A was significantly lower than that of LIN28A negative patients (P<0.05). CONCLUSION:The RNA-binding protein,LIN28A,may promote the occurrence,development and metastasis of breast cancer via EMT,while high expression of LIN28A may affect prognosis of patients with breast cancer.关键词
浸润性乳腺癌/LIN28A/E-钙黏蛋白/波形蛋白/上皮间质转化/凋亡Key words
invasive breast cancer/LIN28A/E-cadherin/vimentin/epithelial-mesenchymal transition/apoptosis分类
医药卫生引用本文复制引用
李幼梅,张思仪,赵连梅,单保恩,张超..LIN28A、 E-cadherin和 vimentin在浸润性乳腺癌中的表达及其临床意义[J].癌变·畸变·突变,2017,29(4):304-308,312,6.基金项目
国家自然科学青年基金项目(81502032) （81502032）
