安徽农业科学2017，Vol.45Issue(16)：3-8,6.
抗水稻细菌性条斑病芽孢杆菌菌株的选育
Study on Biocontrol of Bacterial Leaf Streak with Antagonistic Bacillus
摘要
Abstract
[Objective] The aim was to screen a Bacillus strain with antagonistic ability to bacterial leaf streak and identify,and optimize the fermentation condition to enhance the spore production.[Method] We used Oxford cup method to determine the antagonistic ability;16S rDNA sequence analysis was used to identify the strain;single factor and response surface tests to optimize sporulation conditions.[Result] The JF48 strain belonged to Bacillus amyloliquefaciens,and the diameter of the inhibition zone reached (37 ± 3) mm;the optimal spore culture conditions of Bacillus amyloliquefaciens JF48 were as followsed:corn flour 14.69 g/L,soybean meal 9.54 g/L,CaCl22.O0 g/L,NaCl 5.00 g/L,MgSO4 1.00 g/L,liquid volume 50 mL/250 mL,rotation 200 r/min,initial pH 7.5,temperature 38 ℃,inoculation 1.0％.Under this fermentation condition,the spore production of JF48 strain reached 3.6 × 109 cfu/mL,which was 105 times that of optimizing before.[Conclusion] The strain JF48 has a strong antagonistic ability to bacterial leaf streak and response surface method effectively improves its spore production.关键词
解淀粉芽孢杆菌/细菌性条斑病/响应面法Key words
Bacillus amyloliquefaciens/Bacterial leaf streak/Response surface methodology分类
农业科技引用本文复制引用
吴建飞,张金龙,田迪,朱金梅,张溢峰,白先放..抗水稻细菌性条斑病芽孢杆菌菌株的选育[J].安徽农业科学,2017,45(16):3-8,6.基金项目
2017年度广西高等教育创优计划教学相关项目——优势特色专业项目(优质本科专业). （优质本科专业）
