安徽农业科学2017，Vol.45Issue(16)：9-10,2.
地稔总黄酮含量的测定
Determination of Total Flavonoids from Melastoma dodecandrum Lour.
摘要
Abstract
[Objective] To establish a method for the total flavonoids content determination from Melastoma dodecandrum Lour.[Method] The content of total flavonoids from Melastoma dodecandrum Lour.was determined by UV-visble spectrophotometry with rutin as reference substance,sodium nitrite-aluminum nitrate and sodium hydroxide as chromogenic agent at the detection wavelength of 500 nm.[Result] The rutin showed a good linear relationship at the range of 0.01-0.07 mg/mL(R2 =0.999 8).The average recovery rate was 99.34％,and the relative standard deviation was 0.56％.[Conclusion] The method is simple and reliable,reasonable,specific,reproducible and provides experiment basis for the development of Melastoma dodecandrum Lour..关键词
地稔/总黄酮/紫外-可见分光光度法Key words
Melastoma dodecandrum Lour./Total flavonoids/UV-visble spectrophotometry分类
医药卫生引用本文复制引用
杨菁,朱丹,杜雨涵,陈华巧,于妮..地稔总黄酮含量的测定[J].安徽农业科学,2017,45(16):9-10,2.基金项目
国家级大学生创新创业训练计划项目(责中医大创合字[2015] 12号). （责中医大创合字[2015] 12号）
