安徽农业科学2017，Vol.45Issue(17)：122-123,173,3.
椒样薄荷脱毒苗的规模化生产研究
Study on the Large-scale Production of Virus-free Plantlets of Peppermint
摘要
Abstract
[Objective] To update the peppermint cultivated varieties in Yili area,purify and rejuvenate Yili peppermint.[Method] Shoot tip detoxification,in vitro rapid propagation and mass production of peppermint varieties were conducted by tissue culture technology.Using No.5 pepper as the material,the optimum hormone concentration of peppermint tip differentiation,proliferation and rooting in large scale production was studied.[Result] Sugar and agar bars can be used instead of sucrose and agar to reduce economic costs.In mass production,the optimum proliferation medium of peppermint was 1/2 MS +0.5 mg/L 6-BA +0.01 mg/L NAA,and the optimum rooting medium was 1/2 MS +0.10 mg/L NAA.[Conclusion]The basic medium used in different stages of tissue culture was different,and the hormone combinations were different.The technique of rapid propagation of stem tip can be used to quickly update peppermint varieties.关键词
椒样薄荷/脱毒苗/组织培养/提纯/复壮Key words
Peppermint/Virus-free seedling/In vitro culture/Purify/Rejuvenation分类
农业科技引用本文复制引用
郭丹丽,王自健,蒋新明,路喆,李敏,徐盼盼,王朴..椒样薄荷脱毒苗的规模化生产研究[J].安徽农业科学,2017,45(17):122-123,173,3.基金项目
兵团科技攻关与成果转化项目(2016AC027) （2016AC027）
兵团科技支疆项目(2014AB010) （2014AB010）
师域发展创新支持计划(2015AF011). （2015AF011）
