安徽农业科学2017，Vol.45Issue(18)：115-117,3.
SSR分子标记高效选择抗稻瘟病双基因Pi-1、Pi-2的技术体系
High-efficient Technology System on Marker-assistant Selection of Pi-1 and Pi-2 by SSR
摘要
Abstract
[Objective] Exploring high-efficient detection technology system of SSR molecular markers,to accelerate the process of rice blast resistance breeding and provide a new way of promoting and popularizing the marker-assistant selection technology in enterprise field.[Method] The DNAs of leaves in field were extracted by rapid method,twofold PCR and two-line polyacrylamide gel electrophoresis with primers MRG4766 and AP22 were used to select breeding population.[Result] An technology system with less cost and simple operation was set up to fast detect rice blast resistant genes Pi-1 and Pi-2 accurately and stably on a large scale.[Conclusion] By the technology system,high-efficient marker-assistant selection to the blast resistant breeding population can be achieved to quicken the breeding process,and then popularize marker-assistant selection technology in enterprise field.关键词
分子标记辅助选择/抗稻瘟病/DNA快速提取/两重PCRKey words
Marker-assistant selection/Rice blast resistance/DNA fast extraction/Twofold PCR分类
农业科技引用本文复制引用
周桂林,刘奇燕,范家萌,冯瑞彤,夏泽静,王笑见,王浩波..SSR分子标记高效选择抗稻瘟病双基因Pi-1、Pi-2的技术体系[J].安徽农业科学,2017,45(18):115-117,3.基金项目
国家科技支撑计划项目(2014BAD01B04) （2014BAD01B04）
安徽省重点实验室建设项目(科基[2017]15号). （科基[2017]15号）
