安徽农业科学2017,Vol.45Issue(19):56-57,2.
毒死蜱降解菌的筛选·鉴定·降解特性
Isolation,Identification and Degrading Characteristics of A Chlorpyrifos Degrading Bacteria DC1
摘要
Abstract
[Objective]To screen chlorpyrifos degrading bacteria and understand their characteristics.[Method]A strain capable of utilizing chlorpyrifos as the sole carbon and energy sources was isolated form Chlorpyrifos used paddy soil.[Result]The results showed that the degradation rate of Chlorpyrifos was at 83.3% in liquid culture medium with 15 days.The phylogenetic analysis and 16S rDNA gene sequence analysis were applied to the bacteria classification,the strain was identified as Bacillus subtilis.The phylogenetic analysis also showed that the genetic relationship between strain DC1 and Bacillus tequilensis was propinquity.[Conclusion]The degrading bacteria DC1 was derived from soil and has strong adaptability,and has certain application value for solving soil poisoning ticks residue.关键词
毒死蜱/生物降解/16SrDNA/枯草芽孢杆菌Key words
Chlorpyrifos/Biodegradation/16S rDNA/Bacillus subtilis分类
农业科技引用本文复制引用
罗鑫,张海燕,邵彪,刘明元..毒死蜱降解菌的筛选·鉴定·降解特性[J].安徽农业科学,2017,45(19):56-57,2.基金项目
南通市科技项目(HS2013057). (HS2013057)