安徽农业科学2017，Vol.45Issue(19)：123-125,230,4.
蓼蓝组织培养再生体系的建立
Establishment of Regeneration System for Tissue Culture of Polygonum tinctorium
摘要
Abstract
[Objective]To establish regeneration system for tissue culture of Polygonum tinctorium. [Method]Its seeds, young leaves and stems were used as explants to explore influences of media with different concentration hormone on callus induction, subculture, differentiation and rooting. [Result]Stems of P. tinctorium were ideal explants. The optimum medium for callus induction and subculture was MS+6-BA 1.0 mg/L+2,4-D 2.0 mg/L, the corresponding average induction rate was 73.33%. The optimum medium for callus differentiation was 6-BA 1.0 mg/L +NAA 0.5 mg/L, the corresponding average differentiation rate was 51.67%. The optimum medium for seedlings rooting was 1/2 MS+NAA 0.5 mg/L. The surviving rate after transplanting attained to 100%. [Conclusion]The study provided a new way for P. tinctorium rapid reproduction,andprovided theoretical basis for the preservation and utilization of germplasm resources.关键词
蓼蓝/组织培养/茎段/愈伤组织Key words
Polygonum tinctorium/Tissue culture/Stem/Callus分类
农业科技引用本文复制引用
周江波,陈家鸿,李会会..蓼蓝组织培养再生体系的建立[J].安徽农业科学,2017,45(19):123-125,230,4.基金项目
贵州省科学技术基金项目(黔科合J字〔2014〕2154) （黔科合J字〔2014〕2154）
国家级大学生创新创业训练项目(201510669020) （201510669020）
贵州省教育厅优秀科研创新团队项目(黔教合人才团队字〔2013〕26) （黔教合人才团队字〔2013〕26）
凯里学院院级规划学生课题(〔2014〕16号x1423). （〔2014〕16号x1423）
