安徽医科大学学报2017，Vol.52Issue(6)：781-785,790,6.DOI:10.19405/j.cnki.issn1000-1492.2017.06.001
乳铁蛋白抗菌肽抑制胃癌细胞增殖诱导其凋亡的体外研究
Lactoferrin peptides inhibit the proliferation of gastric cancer cells and induce apoptosis in vitro
摘要
Abstract
Objective To explore the effect of the lactoferrin peptides(LfcinB) on human gastric cancer MGC803 cells proliferation and apoptosis.Methods The proliferation of MGC803 cells at different concentrations of LfcinB were evaluated by CCK-8 assay for 24,48 and 72 h,respectively.Flow cytometry(FCM) was performed to detect the effect of LfcinB on MGC803 cells apoptosis.The expression of apoptotic-specific genes,such as bcl-2,bax and caspase-3 was assessed by RT-PCR and Western blot analysis.Results LfcinB obviously inhibited the proliferation and promoted apoptosis of gastric cancer MGC803 cells (P<0.05,P<0.01) with a time-and dose-dependent manner.LfcinB could markedly inhibit MGC803 cells bcl-2 gene mRNA expression,while promoting bax and caspase-3 mRNA expression,and with the increase of concentration LfcinB that related genes decreased or increased more significantly (P<0.05,P<0.01).Western blot also showed that LfcinB inhibited Bcl-2 protein expression and promoted Caspase-3,Bax protein expression,in line with mRNA results(P<0.05,P<0.01).Conclusion LfcinB can efficiently inhibit proliferation and induce apoptosis of human gastric cancer MGC803 cells through altering the expression of bcl-2,bax and caspase-3 genes.关键词
LfcinB/MGC803/增殖/凋亡Key words
LfcinB/MGC803/proliferation/apoptosis分类
医药卫生引用本文复制引用
杨雪,石皖荣,杨伟杰,何韬,胡阳生,王珊珊,韦文美,秦宜德..乳铁蛋白抗菌肽抑制胃癌细胞增殖诱导其凋亡的体外研究[J].安徽医科大学学报,2017,52(6):781-785,790,6.基金项目
国家自然科学基金(编号:81472448) （编号:81472448）
国家级大学生创新训练项目(编号:201510366035) （编号:201510366035）
安徽医科大学校基金项目(编号:2015xkj048) （编号:2015xkj048）
