安徽医科大学学报2017，Vol.52Issue(6)：819-823,5.DOI:10.19405/j.cnki.issn1000-1492.2017.06.009
抑制Kv1.3表达能够降低人牙周膜成纤维细胞增殖和分化成骨能力
Inhibition expression of Kv1.3 could reduce human periodontal ligament fibroblasts proliferation and osteogenic differentiation capacity
摘要
Abstract
Objective To investigate the value of Kv1.3 on proliferation and osteogenic differentiation capacity in human periodontal ligament fibroblasts(hPDLFs).Methods hPDLFs were isolated and cultured in vitro from normal and periodomtitis,the differential expression of Kv1.3 was evaluated by immunofluorescence,Q-PCR and Western blot.With siRNA silencing Kv1.3 expression,the proliferation and osteogenic differentiation was evaluated in hPDLFs by flow cytometry and MTT.Results Compared with normal controls,Kv1.3 expression was significantly lower in periodontitis hPDLFs by immunofluorescence assay.Western blot results showed that Kv1.3-siRNA could inhibit CyclinD1 and C-myc protein expression(P<0.05);Flow cytometry results showed that Kv1.3-siRNA could significantly inhibit cell G2/M phase;Western blot results showed that Kv1.3-siRNA decreased expression of ALP and OCN.Conclusion The expression of Kv1.3 silencing can inhibit the proliferation and differentiation of osteogenic in hPDLFs significantly.关键词
Kv1.3/人牙周膜成纤维细胞/增殖/分化Key words
Kv1.3/human periodontal ligament fibroblasts/proliferation/differentiation分类
医药卫生引用本文复制引用
朱庆勇,陈文君,朱慧,沈洋,祝心威,蒋勇..抑制Kv1.3表达能够降低人牙周膜成纤维细胞增殖和分化成骨能力[J].安徽医科大学学报,2017,52(6):819-823,5.基金项目
安徽省科技计划项目(编号:12070403062) （编号:12070403062）
