安徽医科大学学报2017，Vol.52Issue(6)：824-828,5.DOI:10.19405/j.cnki.issn1000-1492.2017.06.010
CD4+IL-9+T细胞在实验性动脉粥样硬化中的作用
Role of CD4+IL-9+T cells in experimental atherosclerosis
摘要
Abstract
Objective To investigate the expression and significance of CD4+IL-9+T cells(Th9) in atherosclerosis model of ApoE-/-mice.Methods ApoE-/-mice aged 8 weeks were fed with high fat and their age-matched C57BL/6 control mice were fed with normal diet.The mice were examined at the age of week 8 and week 32.Blood lipid levels in serum were detected.Paraffin sections of aorticroot were used to observe the histological features of atherosclerotic plaques by HE staining.The frequencies of Th9 cells in spleen were measured by flow cytometry.The concentrations of interleukin(IL)-9 and oxidized low density lipoprotein (ox-LDL) in the serum were detected by ELISA assay.Results At the age of week 32,the aortic root of ApoE-/-mice appeared obvious lipid plaques,Th9 cells frequencies and IL-9 concentrations were significantly higher than those of age matched C57BL/6 mice(P<0.01).Th9 cells frequencies and IL-9 concentration were positively correlated with ox-LDL concentration (P<0.01).Conclusion Th9 cells and IL-9 play an important role in the occurrence of atherosclerosis.Th9 cells may become important target of mechanism research and treatment intervention for atherosclerosis.关键词
Th9细胞/白细胞介素-9/动脉粥样硬化/ApoE-/-小鼠Key words
Th9 cells/IL-9/atherosclerosis/ApoE-/-mice分类
医药卫生引用本文复制引用
明婷婷,丁绍威,胡超杰,张翠萍,伍权,李庆..CD4+IL-9+T细胞在实验性动脉粥样硬化中的作用[J].安徽医科大学学报,2017,52(6):824-828,5.基金项目
国家自然科学基金面上项目(编号:81472018) （编号:81472018）
安徽省自然科学基金面上项目(编号:1408085MH145) （编号:1408085MH145）
