安徽医科大学学报2017，Vol.52Issue(6)：828-832,5.DOI:10.19405/j.cnki.issn1000-1492.2017.06.011
高浓度葡萄糖对膀胱癌T24细胞增殖及β-catenin表达的影响
Effects of high concentration of glucose on proliferation and expression of β-catenin in bladder cancer T24 cells
摘要
Abstract
Objective To investigate the effect of high concentration glucose on the proliferation of bladder cancer T24 cells and to explore its possible mechanism.Methods Bladder cancer T24 cells were cultured in DMEM containing 10% fetal bovine serum,which were divided into five groups: blank group(5.5 mmol/L glucose),control group(5.5 mmol/L glucose+25 mmol/L mannitol),glucose groups(10,20,30 mmol/L glucose).Its influence on the cell proliferation was detected by MTT assay and the clonogenic experiment.The expression of β-catenin mRNA levels was determined by real-time quantitative PCR.Western blot assay was used to detect β-catenin protein expression.Results Compared with the blank and control group,MTT assay and the clonogenic experimen showed that high glucose significantly enhanced the proliferation of T24 cells.The results of Western blot assay showed that high glucose promoted the expression of β-catenin protein in T24 cells.Real-time quantitative PCR results showed that high glucose promote mRNA of β-catenin gene in T24 cells.Conclusion High concentration of glucose significantly promoted the proliferation of bladder cancer T24 cells and promoted the expression of β-catenin protein and its mRNA in T24 cells.The mechanism underlying may be related to the up-regulation of β-catenin expression.关键词
高糖环境/膀胱癌/增殖/T24/β-cateninKey words
glucose/bladder cancer/proliferation/T24/β-catenin分类
医药卫生引用本文复制引用
何小龙,钟珍教,何笑云,朱海琴,徐方明,高漓,张天禹,蒋雷鸣..高浓度葡萄糖对膀胱癌T24细胞增殖及β-catenin表达的影响[J].安徽医科大学学报,2017,52(6):828-832,5.基金项目
国家自然科学基金(编号:81360377、81660425) （编号:81360377、81660425）
广西自然科学基金项目重点项目(编号:2015jjDA40034) （编号:2015jjDA40034）
桂林医学院大学生创新创业训练计划项目(编号:201610601040) （编号:201610601040）
