安徽医科大学学报2017，Vol.52Issue(6)：850-854,5.DOI:10.19405/j.cnki.issn1000-1492.2017.06.016
酢浆草遗传多样性的ISSR分析
ISSR analysis on genetic diversity of Oxalis
摘要
Abstract
Objective To study the genetic diversity of germplasm resources of Oxalis corniculata,and to provide molecular basis for reference of Oxalis corniculata by assessing genetic differentiation and selecting of excellent germplasm resources.Methods The genetic diversity with 36 samples of 18 populations of Oxalis corniculata and 4 samples of 2 populations of Oxalis corymbosa was analyzed by ISSR molecular marker technique.Results 10 primers were selected from 59 ISSR primers which amplified 89 DNA bands,and 78 bands among them were polymorphic which the percentage of polymorphic bands was 87.64%,the average DNA bands amplified by each primer were 8.9 bands.The average number of effective allele was 1.388 8 of 40 samples,the Nei''s gene diversity and Shannon''s information index were 0.349 3 and 0.225 2 by POPGENE1.32 and NTSYS-pc software.These 40 samples were clustered into 4 groups at the genetic similarity coefficient 0.655 8 by UPGMA.Conclusion The genetic diversity in 40 samples of Oxalis corniculata collected in this paper isrich,and there were significant differences in genetic diversity of ISSR between Oxalis corniculata and Oxalis corymbosa.关键词
酢浆草/ISSR/遗传多样性Key words
Oxalis corniculata L./ISSR/genetic diversity分类
医药卫生引用本文复制引用
吴林菁,冯华,杜强,吴红梅,徐锋,杨烨,王祥培,沈祥春..酢浆草遗传多样性的ISSR分析[J].安徽医科大学学报,2017,52(6):850-854,5.基金项目
贵州省中药现代化科技产业研究开发专项项目(编号:黔科合中药字[2010]5028号) （编号:黔科合中药字[2010]5028号）
遵义市创新人才团队培养项目(编号:遵市科合[2016]7号) （编号:遵市科合[2016]7号）
贵州省科技合作项目(编号:黔科合LH字[2016]7368) （编号:黔科合LH字[2016]7368）
贵州省普通高等学校创新团队建设项目(编号:黔教合人才团队字[2014]31) （编号:黔教合人才团队字[2014]31）
贵州省科技创新人才团队建设项目(编号:黔科合人才团队[2015]4025号) （编号:黔科合人才团队[2015]4025号）
贵州省高层次创新型人才培养项目(编号:黔科合人才[2015]4029号) （编号:黔科合人才[2015]4029号）
贵州省大学生创新创业训练计划项目(编号:201610660031) （编号:201610660031）
