安徽医科大学学报2017，Vol.52Issue(7)：1008-1011,4.DOI:10.19405/j.cnki.issn1000-1492.2017.07.014
人肝细胞癌中miR-23a和XIAP的表达及意义
Expression of miR-23a and XIAP in hepatocellular carcinoma and their significance
摘要
Abstract
Objective To investigate the expression and correlation of miR-23a and X-linked inhibitor of apoptosis protein(XIAP) in hepatocellular carcinoma(HCC) and their clinicopathological significance.Methods The expressions of miR-23a in 150 HCC and 136 adjacent tissues were detected by in situ hybridization.The expressions of XIAP and Ki-67 protein were detected by immunohistochemistry two-step method.Results The high expression rates of miR-23a were 48.7%(73/150) and 77.2%(105/136) in HCC tissues and adjacent non-cancerous tissues respectively,there was significant difference between the two groups(P=0.001).The high expression rates of XIAP were 25.3%(38/150) and 22.1%(30/136) in HCC tissues and adjacent non-cancerous tissues respectively, there was no significant difference between the two groups.The expression rate of miR-23a was higher in male than in female(P=0.037),HBsAg positive was higher than that of HBsAg negative patients(P=0.022), large hepatocellular carcinoma was higher than that of small hepatocellular carcinoma(P=0.021), and stageⅠ~Ⅱwas higher than that of stageⅢ~Ⅳ(P=0.002).While the high expression of XIAP protein in patients with small hepatocellular carcinoma was higher than that of large hepatocellular carcinoma(P=0.001), theⅠ~Ⅱ stage was higher than Ⅲ~Ⅳstage(P=0.009).In addition,both of them had no correlation with age, liver cirrhosis and histological grading.The expression of miR-23a and XIAP protein in hepatocellular carcinoma was negatively correlated by Pearson′s correlation(rs=-0.308,P<0.01).Conclusion miR-23a and XIAP may be involved in the development and progression of HCC.关键词
肝细胞癌/miR-23a/XIAP/免疫组化/原位分子杂交Key words
HCC/miR-23a/XIAP/immunohistochemistry/in situ hybridization分类
医药卫生引用本文复制引用
马伟,吴文涌,吴正升,汪树静,余昌俊..人肝细胞癌中miR-23a和XIAP的表达及意义[J].安徽医科大学学报,2017,52(7):1008-1011,4.基金项目
国家自然科学基金(编号:81572305) （编号:81572305）
