安徽医科大学学报2017，Vol.52Issue(7)：1073-1077,5.DOI:10.19405/j.cnki.issn1000-1492.2017.07.030
HepG2.2.15细胞的差异性表达及对IFN-α的反应性研究
Differential expressions of HBsAg and HBeAg by HepG2.2.15 cells and their reactivity to IFN-α
摘要
Abstract
10 single-cell clones of HepG2.2.15 cells were isolated by limited dilution and their expressions of HBsAg and HBeAg were determined by ELISA.According to expressions of HBsAg and HBeAg, the highest, medium and the lowest-expression clones were selected.Cytotoxicity of IFN-α were analyzed by MTT assay and the results suggested that IFN-α showed similar cytotoxicity to the different clonal cell lines.The clones were treated with IFN-α and HBsAg and HBeAg expressions were determined by ELISA.IFN-α weakly inhibited HBeAg expression and strongly inhibited HBsAg expression and HBV DNA replication, and the inhibition effect was stronger for those with higher expression levels.HBsAg and HBeAg were differentially expressed in HepG2.2.15 cells and the highly expressed clones were more sensitive to IFN-α.关键词
HepG2.2.15/乙型肝炎病毒/IFN-αKey words
HepG2.2.15/HBV/IFN-α分类
医药卫生引用本文复制引用
汤波,韦佳佳,徐丙发,范鲁雁..HepG2.2.15细胞的差异性表达及对IFN-α的反应性研究[J].安徽医科大学学报,2017,52(7):1073-1077,5.基金项目
国家自然科学基金(编号:81470165) （编号:81470165）
