安徽医科大学学报2017，Vol.52Issue(8)：1123-1128,6.DOI:10.19405/j.cnki.issn1000-1492.2017.08.006
敲减长链非编码RNA ATB抑制胶质瘤细胞增殖、迁移和侵袭
ATB knockdown inhibits the proliferation,migration and invasion of glioma cells
摘要
Abstract
Objective To investigate the effects of long non-coding RNA activated by TGF-β(ATB) on the proliferation, migration and invasion of human U87glioma cell line.Methods The expression of ATB in human U87 glioma cell line and human normal astrocyte(HEB) were detected by real-time quantitative PCR(qRT-PCR).And based on this, shRNA ATB plasmid were constructed ,which could knockdown ATB expression after transfected into U87 cells.The proliferation ability of cell was investigated through MTT assay and the colony-formation ability was tested by the cell cloning assay.Cell migration and invasion were measured by non-Matrigel transwell assay and Matrigel transwell assay in vitro.Results Using qRT-PCR analysis, we found ATB expression levels in U87 cell line was significantly increased compared with HEB(P<0.01).The expression of ATB was remarkable reduced after U87 transfected with shRNA-ATB plasmid(the experimental group) compared with U87 cells transfected with shRNA control(the control group).Cloning formation experiment indicated that colony-formation ability was decreased than that of control group(P<0.01).MTT assay indicated that the number of viable cell was lower than that of control group(P<0.01).In addition, Transwell assay further verified that knockdown of ATB significantly inhibited the migration and invasion ability of U87 cells(P<0.01).Conclusion ATB knockdown inhibit glioma cell proliferation, migration and invasion, which indicates that ATB may represent a potential therapeutic target for the treatment of human glioma.关键词
胶质瘤/长链非编码RNA/ATB/细胞增殖/细胞迁移/细胞侵袭Key words
glioma/long non-coding RNA/ATB/cell proliferation/cell migration/cell invasion分类
医药卫生引用本文复制引用
张新雨,马春春,卞尔保,王洪亮,宗钢,赵兵..敲减长链非编码RNA ATB抑制胶质瘤细胞增殖、迁移和侵袭[J].安徽医科大学学报,2017,52(8):1123-1128,6.基金项目
国家自然科学基金青年基金(编号:81402078、81502149) （编号:81402078、81502149）
安徽省自然科学基金项目(编号:1608085MH225、1508085MH194) （编号:1608085MH225、1508085MH194）
安徽医科大学校科研基金(编号:2015xkj021) （编号:2015xkj021）
