北京林业大学学报2017，Vol.39Issue(6)：13-21,9.DOI:10.13332/j.1000-1522.20170034
胡杨PeAPY1和PeAPY2调控拟南芥耐盐机制研究
PeAPY1 and PeAPY2 of Populus euphratica regulating salt tolerance in Arabidopsis thaliana
摘要
Abstract
We investigated the effects of Populus euphratica apyrase genes ( PeAPY1 and PeAPY2 ) on salt tolerance of higher plants. Using PeAPY1 and PeAPY2 transgenic plants, Atapy1 and Atapy2 mutants, Arabidopsis wild type ( WT) and vector controls ( VC) , we examined the effects of NaCl on root length, relative electrolyte leakage, cell viability, H2O2 , extracellular ATP ( eATP ) and antioxidant enzyme activities. Results showed that the low salinity ( 50 mmol/L NaCl ) had no significant effects on root growth, physiological and biochemical parameters. However, a high salinity ( 100 mmol/L NaCl ) markedly reduced root length, cell viability and activities of antioxidant enzymes ( superoxide dismutase /SOD, ascorbate peroxidase /APX, catalase /CAT ) . In contrast, the high NaCl increased relative electrolyte leakage, H2O2 , and eATP levels in all the tested lines. PeAPY1/2-transgenic lines exhibited a less pronounced effects as compared with the Arabidopsis Atapy mutants under high salinity. We inferred that the overexpression of PeAPY1/2 led to an up-regulated apyrase, which hydrolyzed eATP and down-regulated the ATP induction of H2O2 . Moreover, PeAPY1/2 -transgenic lines maintained activity of antioxidant enzymes under salt stress. This would reduce the membrane peroxidation induced by the reactive oxygen species. As a result, the membrane integrity and cell viability were less affected by salt stress, leading to an increased salt tolerance in PeAPY1/2-transgenic plants.关键词
PeAPY1/PeAPY2/盐胁迫/膜透性/胞外ATP/H2O2Key words
PeAPY1/PeAPY2/salt stress/membrane leakage/eATP/H2O2分类
农业科技引用本文复制引用
邓佳音,张艳丽,张一南,赵瑞,李金克,周晓阳,刘香芬,陈少良..胡杨PeAPY1和PeAPY2调控拟南芥耐盐机制研究[J].北京林业大学学报,2017,39(6):13-21,9.基金项目
国家自然科学基金项目(31270654、31570587)、教育部科学技术研究(科学技术类)项目(113013A)、北京市自然科学基金项目(6172024)、高等学校学科创新引智计划项目(111project,B130007)、教育部创新团队发展计划项目(IRT13047). （31270654、31570587）
