吉林大学学报（医学版）2017，Vol.43Issue(4)：679-684,6.DOI:10.13481/j.1671-587x.20170403
灵芝多糖硫酸酯对大鼠脑缺血再灌注损伤的保护作用及其机制
Protective effect of Ganodermalucidum polysaccharide sulfate on cerebral ischemia reperfusion injury in rats and its mechanism
摘要
Abstract
Objective:To modify Ganodermalucidum polysaccharides(GLP) with sulfate and observe the protective effect of Ganodermalucidum polysaccharide sulfate (GLPS) on the cerebral ischemia reperfusion injury in the rats,and to investigate its mechanism.Methods:GLP was modified by sulfation to obtain GLPS.A total of 100 SD rats were randomly divided into sham operation group, model group, GLP group (40 mg·kg-1·d-1), GLPS group (40 mg·kg-1·d-1) and nimodipine group (1 mg·kg-1·d-1).The cerebral ischemia reperfusion models were established by middle cerebral artery occlusion method in the rats.The neurologic deficit score and the content of water in brain tissue of the rats with cerebral ischemia reperfusion injury were detected and the activities of superoxide dismutase(SOD) and the levels of malondialdehyde (MDA) were detected.The levels of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB),tumor necrosis factor-alpha (TNF-α), interleukin-1 (IL-1) and interleukin-6 (IL-6) in the brain tissue homogenate were detected by ELISA.Western blotting method was used to detect the protein expression levels of HSP70 and p-Akt in the brain tissue of the rats.Results:Compared with model group, the neurological function scores of the rats in GLP group and GLPS group were decreased(P<0.01),the water contents in brain tissue were decreased(P<0.05), the SOD activities were increased and the MDA levels were decreased(P<0.05), and the levels of NF-κB, TNF-α, IL-1 and IL-6 were decreased(P<0.05);the effect in GLPS group was significantly better than that in GLP group(P<0.05).The results of Western blotting method showed that the p-Akt protein expression levels in the brain tissue of the rats in GLP and GLPS groups were increased compared with model group (P<0.05);compared with model group, the HSP-70 protein expression level in the brain tissue of the rats in GLPS group was increased(P<0.01),but the effect in GLP group was not obvious.Conclusion:Sulfation can significantly improve the protective effect of GLP on the cerebral ischemia reperfusion injury in the rats and its mechanism may be related to regulating the HSP70/PI3K/Akt signaling pathway and inhibiting the inflammatory reaction damage to the nerve cells of reperfusion.关键词
灵芝多糖/硫酸酯化/脑缺血再灌注损伤/信号通路Key words
Ganodermalucidum polysaccharide/sulfation/cerebral ischemia reperfusion injury/signaling pathway分类
医药卫生引用本文复制引用
李亚巍,韩丽琴,金瑛,朱文赫..灵芝多糖硫酸酯对大鼠脑缺血再灌注损伤的保护作用及其机制[J].吉林大学学报（医学版）,2017,43(4):679-684,6.基金项目
国家自然科学基金资助课题(21102055) （21102055）
吉林省科技厅科技发展计划项目资助课题(20140204002YY) （20140204002YY）
吉林省吉林市科技计划项目资助课题(20156428) （20156428）
吉林省卫计委青年科研项目资助课题 (2015Q041) （2015Q041）
