吉林大学学报（医学版）2017，Vol.43Issue(4)：685-689,前插1,6.DOI:10.13481/j.1671-587x.20170404
杨梅素对人卵巢癌SKOV3细胞自噬的诱导作用及线粒体分裂的促进作用
Induction effect of myricetin on autophagy in SKOV3 cells and promoting effect on mitochondrial fission
摘要
Abstract
Objective:To observe the effects of myricetin on autophagy and mitochondrial fission in the human ovarian cancer SKOV3 cells,and to explore its induction effect on autophagy and promoting effect on mitochondrial fission.Methods: The SKOV3 cells were cultured in vitro.0,20,40,and 60 g·L-1 myricetin were used in control group and low, middle, high doses of myricetin groups for 12 h.The changes of mitochondrial membrane potential were detected by flow cytometry;the morphology of mitochondria was observed by MitoTracker Red;the expression levels of dynamin related protein1 (Drp1) and fission 1 (Fis1) in various groups were detected by Western blotting method;and the expression levels of autophagy related protein LC3 were detected by both Western blotting method and immunofluorescence method.Results:Compared with control group, the ratios of decreased mitochondria in different doses of myricetin groups were significantly increased(t=3.27, t=6.85, t=5.49,P<0.05).Compared with control group, the numbers of mitochondria in different doses of myricetin groups were increased, and the mitochondria looked more like gravel.Compared with control group,the expression levels of Drp1 in different doses of myricetin groups were significantly increased (t=4.35, t=3.28, t=6.17,P<0.05), and the expression levels of Fis1 were increased also(t=8.32, t=6.74, t=9.27).The immunofluorescence results showed that the expression levels of LC3 in different doses of myricetin groups were significantly increased with the increase of myricetin dose compared with control group.The Western blotting results showed that the ratios of LC3-Ⅱ/LC3-Ⅰ in middle and high doses of myricetin groups were significantly increased compared with control group(t=3.28, t=4.21,P<0.05).Conclusion: Myricetin can induce the autophagy of SKOV3 cells, and it can also promote the mitochondrial fission.关键词
杨梅素/SKOV3细胞/自噬/线粒体分裂Key words
myricetin/autophagy/mitochondrial fission/SKOV3 cells分类
医药卫生引用本文复制引用
于洋,刘师兵,李松岩,徐路,徐冶..杨梅素对人卵巢癌SKOV3细胞自噬的诱导作用及线粒体分裂的促进作用[J].吉林大学学报（医学版）,2017,43(4):685-689,前插1,6.基金项目
国家自然科学基金面上项目资助课题(81372793) （81372793）
吉林省教育厅"十二五"科技研究重点项目资助课题(吉教科合字[2016]第237号) （吉教科合字[2016]第237号）
