吉林大学学报（医学版）2017，Vol.43Issue(4)：743-746,4.DOI:10.13481/j.1671-587x.20170416
Hiwi基因靶向沉默对乳腺癌MCF-7/ADM细胞化疗敏感性的影响
Influence of Hiwigene targeting silence in chemotherapy sensitivity of breast cancer MCF-7/ADM cells
摘要
Abstract
Objective:To explore the changes of chemotherapy sensitivity of breast cancer MCF-7/ADM cells after Hiwi gene targeting silence, and to illuminate the role of Hiwi gene in resistant mechanism of breast cancer MCF-7/ADM cells.Methods:The MCF-7/ADM cells were transfected with Hiwi control,siRNA1 Hiwi gene and siRNA2 Hiwi gene as Hiwi control group, Hiwi siRNA1 group and Hiwi siRNA2 group.The expression levels of Hiwi gene mRNA and protein in breast cancer MCF-7/ADM cells were detected by Real-time PCR and Western blotting methods to judge the transfection rates in various groups.After transfection,the breast cancer MCF-7/ADM cells in various groups were treated with the different concentrations(0,0.1,0.5 and 1.0 mg·L-1) of adriamycin, and the cell resistant sensitivities were detected by MTT method.Results:Compared with control group(0 mg·L-1 adriamycin), the expression levels of Hiwi gene mRNA and protein inMCF-7/ADM cells in Hiwi siRNA1 and Hiwi siRNA2 groups were significantly decreased (P<0.01).Compared with control group, the survival rates of Cells in Hiwi siRNA1 and Hiwi siRNA2 groups were significantly decreased after treated with different concentrations of adriamycin,and the survival rates in Hiwi siRNA1 and Hiwi siRNA2 groups were significantly decreased(P<0.01);they were (48.15±6.28)% and (41.73±6.17)% when the concentration of adriamycin was 1 mg·L-1,and the sensitivities to the adriamycinwere obviously enhanced(P<0.01).Conclusion:The target silencing Hiwi genes in MCF-7/ADM cells is efficient.The sensitivity of MCF-7/ADM cells to adriamycin restores after Hiwi gene silencing.关键词
乳腺肿瘤/Hiwi基因/靶向沉默/MCF-7/ADM细胞/敏感性Key words
breast neoplasms/Hiwigene/targeted silence/MCF-7/ADM cells/sensitivity分类
医药卫生引用本文复制引用
任爱华,王大伟..Hiwi基因靶向沉默对乳腺癌MCF-7/ADM细胞化疗敏感性的影响[J].吉林大学学报（医学版）,2017,43(4):743-746,4.基金项目
吉林省教育厅"十三五"科学技术研究项目资助课题(吉教科合字[2016]第67号) （吉教科合字[2016]第67号）
