吉林大学学报（医学版）2017，Vol.43Issue(4)：747-751,5.DOI:10.13481/j.1671-587x.20170417
氟环境下变形链球菌耐氟菌株ciaH、eno和pykF基因的差异表达及其意义
Differential expressions of ciaH, eno and pykF genes of fluoride-resistant Streptococcus mutans cultivated in fluoride environment and its significance
摘要
Abstract
Objective:To investigate the relationship between ciaH, eno, pykF genes and fluoride resistance through determining the differential expressions of ciaH, eno and pykF genes of fluoride-resistant Streptococcus mutans cultivated in fluoride environment. Methods:The cultured Streptococcus mutans and their fluoride-resistant strains were divided into UA (Streptococcus mutans subcultured in BHI without NaF), FR (fluoride-resistant Streptococcus mutans subcultured in BHI without NaF) and FFR (fluoride-resistant Streptococcus mutans subcultured in BHI containing 1 g·L-1 NaF) groups.After 11 h (logarithmic phase) and 20 h (platform stage) cultivation, the expression levels of ciaH, eno and pykF mRNA were detected by RT-PCR method.Results:Compared with FR group, the expression levels of ciaH, eno and pykF mRNA in FFR group were increased both in the logarithmic phase and the platform stage(P<0.05 or P<0.01).Compared with UA group, the expression levels of eno and pykF mRNA in FR group were decreased both in the logarithmic phase and the platform stage(P<0.01), whereas the expression level of ciaH mRNA had no significant difference in the logarithmic phase (P>0.05), but it was increased in the platform stage (P<0.01).Conclusion:Fluoride can increase the expression levels of ciaH, eno and pykF genes in fluoride-resistant Streptococcus mutans, indicating that these genes are related to the production of fluoride resistance.关键词
ciaH/eno/pykF/变形链球菌/变形链球菌耐氟菌株/氟抗性Key words
ciaH/eno/pykF/Streptococcus mutans/fluoride-resistant Streptococcus mutans/fluoride resistance分类
医药卫生引用本文复制引用
张琦,赵洪岩,张志民,张红,李文月,超博,杨瑶瑶..氟环境下变形链球菌耐氟菌株ciaH、eno和pykF基因的差异表达及其意义[J].吉林大学学报（医学版）,2017,43(4):747-751,5.基金项目
吉林省科技厅自然科学基金资助课题(20160101003JC) （20160101003JC）
