草业学报2017，Vol.26Issue(6)：111-119,9.DOI:10.11686/cyxb2016442
结缕草肉桂醇脱氢酶基因家族全基因组序列鉴定和表达分析
Genome-wide identification and characterization of the cinnamyl alcohol dehydrogenase gene family in Zoysia japonica
摘要
Abstract
Cinnamyl alcohol dehydrogenase (CAD,EC1.1.1.195) is a key enzyme in the lignin biosynthesis pathway.By using NADPH as a cofactor,CAD catalyzes cinnamyl aldehydes to alcohols and plays an important role in plant development and stress responses.This study identified 16 CAD genes in Zoysia japonica.Phylogenetic and structural analyses of these genes were conducted.Semi-quantitative RT-PCR indicated that 5 ZjCAD genes were specifically expressed in leaves.Lignin histochemical staining and expression analyses of ZjCAD genes in different cultivars and a wild accession further showed that the expression pattern of ZjCAD6 was positively correlated with leaf lignin contents.These results indicate that ZjCAD6 may be the key gene that regulates lignin biosynthesis in the leaves of Z.japonica.The full length of the ZjCAD6 gene is 2736 bp,and is comprised of three introns and four exons.The 1074 bp open reading frame of ZjCAD6 encodes 357 amino acids.ZjCAD6 protein contains two Zn2+-binding motifs and a NADP(H)-binding motif,which is vital for its catalytic activity.Our results provide an important reference for further study of both lignin content's molecular mechanisms and the tensile strength of Z.japonica leaves.关键词
结缕草/木质素/肉桂醇脱氢酶/生物信息学分析/基因表达Key words
Zoysia japonica/lignin/cinnamyl alcohol dehydrogenase/bioinformatics analysis/gene expression引用本文复制引用
史经昂,张兵,肖晓琳,马晶晶,杨向阳,刘建秀..结缕草肉桂醇脱氢酶基因家族全基因组序列鉴定和表达分析[J].草业学报,2017,26(6):111-119,9.基金项目
江苏省公益院所能力提升项目一草坪种质创新(BM2015019-1),江苏省体育产业引导项目“江苏省运动草坪研究中心建设暨运动草坪标准化项目”和江苏省体育产业引导项目“天然草坪耐践踏性关键技术体系研发与集成示范”资助. （BM2015019-1）
