草业学报2017，Vol.26Issue(6)：145-152,8.DOI:10.11686/cyxb2016293
防治紫花苜蓿真菌病害的生防菌筛选
Screening of inhibitory bacteria for prevention and treatment of fungal diseases of Medicago sativa
摘要
Abstract
The inhibitory effect of two bacteria on 9 alfalfa pathogens were tested on PDA medium using the confrontation method;the taxonomic status of each pathogen species were determined using morphological and molecular criteria.The bacteria identified were Bacillus amyloliquefaciens and Streptomyces kanamycin.The inhibitory effect of Bacillus amyloliquefaciens ranged from 24.02％-71.11％,with the greatest effect shown against Cercospora medicaginis (71.11％),Stemphylium botryosum (64.76％) and Fusarium roseum (60.55％).The inhibitory range of Streptomyces kanamycin was similar (49.95％-76.90％) with greatest effect against Colletotrichum trifolii (76.90％),Phoma medicaginis (70.94％),Stem ph ylium botryosum (68.01 ％) and Leptosphaerulina briosiana (60.99 ％).Bacillus amyloliquefaciens and Streptomyces kanamycin showed varying degrees of antagonism to different alfalfa pathogenic fungi indicate that they have promise as biological control agents for a variety of Medicago sativa foliar diseases,suggesting further development should be undertaken.关键词
紫花苜蓿/病原真菌/拮抗/鉴定/解淀粉芽孢杆菌/卡那霉素链霉菌Key words
Medicago sativa/pathogenic fungi/antagonistic/identification/Bacillus amyloliquefaciens/Streptomyces kanamycin引用本文复制引用
胡进玲,汪治刚,徐娜,党淑钟,李彦忠..防治紫花苜蓿真菌病害的生防菌筛选[J].草业学报,2017,26(6):145-152,8.基金项目
国家自然科学基金(No.31272496),公益性行业(农业)科研专项经费项目(No.201303057),国家牧草产业技术体系(CARS-35)和云南省高端科技人才引进项目(2012HA012)资助. （No.31272496）
