草业学报2017，Vol.26Issue(6)：153-167,15.DOI:10.11686/cyxb2016498
解淀粉芽孢杆菌复合菌剂对玉米秸秆的降解作用及表征
Analysis of the degradation of corn stalk fermented by complex bacteria composed of two Bacillus amyloliquefaciens strains
摘要
Abstract
In order to obtain a complex bacteria agent that can be used in the fermentation of corn stalk and improve its quality as silage,two Bacillus amyloliquefaciens strains with high lignocellulose-degrading ability were mixed to make a complex agent whose degradation effect could be studied.The microstructure and degradation products of corn stalk inoculated with the complex bacteria agent were analyzed using FTIR,1H-NMR,SEM and GC/MS.Fermentation tests showed that the microbial agent efficiently degraded lignocellulose in corn straw.When the agent was inoculated for 24 days,the degradation rate of lignin,cellulose and hemicellulose reached 48.4％,30.5％ and 41.4％ respectively.FTIR and 1H-NMR spectrum analysis showed that the main linking covalent bonds,such as β-O-4 and β-β linkages between the lignin monomers,the bonds connecting the lignin to cellulose and the carbohydrate bonds in lignocellulose's molecular structure,were all broken.The complete destruction of plant tissue structure was also observed with electron microscope scanning.GC/MS analysis of low-molecule substances in the fermented corn stalk showed that the lignin was degraded into small molecular aromatic compounds,including amphetamine and phenylpropionic acid,monomers produced by the depolymerization of lignin,and benzyl alcohol and benzoic acid esters,which are degradation products of lignin phenyl propane monomers.GC/MS analysis of carbohydrate degradation products showed that the complex bacteria could not only convert cellulose,hemicellulose and other macromolecule carbohydrates into reducing monosaccharides,such as glucose,xylose,mannose and galactose,but that it could also metabolize these monosaccharides into glycol,glycerol and short-chain fatty acids.In conclusion,these results show that the complex bacteria agent effectively degrades lignocellulose and could be widely used to convert corn stalk into high quality forage.关键词
解淀粉芽孢杆菌/复合菌剂/玉米秸秆/降解/表征Key words
Bacillus amyloliquefaciens/complex bacteria/corn stalk/degradation/characterization引用本文复制引用
李红亚,李文,李术娜,王树香,李猛,田苗苗,朱宝成..解淀粉芽孢杆菌复合菌剂对玉米秸秆的降解作用及表征[J].草业学报,2017,26(6):153-167,15.基金项目
河北省科技支撑计划项目(12226605)和河北省教育厅科学技术研究项目(2011232)资助. （12226605）
