川北医学院学报2017，Vol.32Issue(3)：339-343,5.DOI:10.3969/j.issn.1005-3697.2017.03.007
嗜酸乳酸杆菌对实验性溃疡性结肠炎治疗的最佳浓度探索及免疫学机制初探
The appropriate concentration of Lactobacillus acidophilus and immunological mechanisms for the treatment of UC
摘要
Abstract
Objective:To compare the effect of different concentration of Lactobacillus acidophilus (L.acidophilus) on ulerative colitis (UC),and to analysis the influenceof of proximal and distal colon of bacteria,to find the appropriate concentration of L.acidophilus for UC,and to explore the immunological mechanism.Methods:56 female Balb/c mice were randomly divided into 7 groups (n=8),dextran sulfate sodium (DSS) made of UC model rats were given different concentration of L.acidophilus, hormones,physiological saline intervention UC model rats,observed the general condition of the mice,calculated the disease activity index (DAI) score,tissue injury score,the intestinal flora of mice was taken for intestinal flora analysis,and PCR and Western-Blotting were used to detect UC related IL-23,IL-17,TNF-α.Results:In the 106CFU/mL concentration group,the UC symptoms relief of the L.acidophilus intervention group were the most obvious,and the number of probiotics (lactobacillus and bifidobacteria) in the distal colon was the most,and the pathogen (staphylococcus aureus) was the lowest.UC related IL-23,IL-17,TNF-α regardless of mRNA level or protein level,were lowest in the L.acidophilus intervention group,especially in the 106 U /10 g L.acidophilus gavage group.Conclusion:L.acidophilus UC and its treatment in the distal colon have higher concentrations of lactic acid bacteria,but the result is not completely positive correlation with the concentration of bacteria,inflammatory factor IL-23,IL-17 and TNF-α are positively correlated with the UC disease severity,associate with the therapeutic effect of L.acidophilus on UC.关键词
嗜酸乳酸杆菌/菌群分析/实验性结肠炎/免疫学机制/白细胞介素23/白细胞介素17Key words
Lactobacillus acidophilus/Flora analysis/Experimental colitis/Immunological mechanism/Interleukin 23/Interleukin 17分类
医药卫生引用本文复制引用
陈琳琳,郑倩..嗜酸乳酸杆菌对实验性溃疡性结肠炎治疗的最佳浓度探索及免疫学机制初探[J].川北医学院学报,2017,32(3):339-343,5.基金项目
国家自然科学基金青年科学基金项目(8160042) （8160042）
