磁共振成像2017，Vol.8Issue(7)：526-531,6.DOI:10.12015/issn.1674-8034.2017.07.009
利用扩散加权成像评估MitoQ对大鼠肾脏缺血再灌注损伤的保护作用
MitoQ protects rodent kidneys from ischemia-reperfusion injury: observations with DWI
摘要
Abstract
Objective: To investigate the effect of the mitochondria targeted antioxidant MitoQ in protecting from renal ischemia-reperfusion injury (IRI) in rats by longitudinal observation on apparent diffusion coefficient (ADC) values of the kidney measured with diffusion-weighted imaging (DWI). Materials and Methods: Renal IRI was induced by temporarily clamping the left renal artery for 45 minutes and then reperfusion was realized. Ten male Sprague–Dawley rats were randomly divided into two groups: MitoQ group (n=5, IRI+MitoQ) and control group (n=5, IRI+saline). DWI was performed just before the operation (day 0) and on day 2, 5, 7 and 14 after the operation. ADC value of the outer stripe of the outer medulla (OSOM) was measured on bilateral kidneys. Renal histopathology damage score was evaluated after the final MRI examination. Inter- and intra- group differences were assessed using the Least Significant Difference (LSD). Kruskal-Wallis H test and Mann-Whitney U test were used for histopathology damage score analysis. Results: The ADC values of the kidneys in two groups on day 0 had no statistical significance. The ADC values of the left OSOM dropped dramatically after IRI in both of the groups, and it was more obvious in control group. The ADC values of the OSOM on IRI kidneys were lower than their counterparts (P<0.01), and the ADC values of the OSOM in control group were even lower than that in MitoQ group Day 2, the left OSOM on MitoQ group and control group: (3.66±0.29)×10-4 mm2/s. (3.09±0.39)×10-4 mm2/s, P<0.05. Day 5, the left OSOM on MitoQ group and control group: (3.75±0.32)×10-4 mm2/s, (2.95±0.79)×10-4 mm2/s, P<0.05. Day 7, the left OSOM on MitoQ group and control group: (3.77±0.42)×10-4 mm2/s, (2.98±0.49)×10-4 mm2/s, P<0.05, Day 14, the left OSOM on MitoQ group and control group: (3.93±0.23)×10-4 mm2/s, (3.05±0.20)×10-4 mm2/s, P<0.05. Renal histopathology analysis showed that renal damage was the most predominant on the OSOM of IRI kidneys in control group, whose histopathology damage scores were significantly higher than those in MitoQ group (P<0.01). Conclusions: DWI could be a noninvasive method to evaluate the effect of MitoQ on reducing renal IRI in rats.关键词
肾脏/缺血再灌注损伤/弥散磁共振成像Key words
Kidney/Ischemia-reperfusion injury/Diffusion magnetic resonance imaging分类
医药卫生引用本文复制引用
刘晓鸽,吴焕焕,张蕊,孙浩然..利用扩散加权成像评估MitoQ对大鼠肾脏缺血再灌注损伤的保护作用[J].磁共振成像,2017,8(7):526-531,6.基金项目
国家自然科学基金(编号:81171316) This work was part of National Natural Science Foundation of China (No. 81171316). （编号:81171316）
