重庆医学2017，Vol.46Issue(19)：2599-2603,5.DOI:10.3969/j.issn.1671-8348.2017.19.003
低氧培养对小鼠胚胎成纤维细胞生长和线粒体分布及功能的影响
Effects of hypoxia on the growth, mitochondria distribution and function of mouse embryonic fibroblast
摘要
Abstract
Objective To explore the effects of hypoxia on the growth,mitochondria distribution and function of mouse embryonic fibroblasts(MEFs).Methods MEFs were sub-cultured in the hypoxia group containing 5％ oxygen and normal oxygen group containing 20％ oxygen,every 24 hours,living MEFs were counted by using trypan blue staining.Mito-Tracker Green was used to stain mitochondria,then cells were observed by using laser confocal microscope.The ATP kit was used to detect ATP synthesis.Results During the logarithmic phase,the numbers of living cells in the hypoxia group were higher than those in the normal oxygen group,the differences were statistically significant (P＜0.05).The percentages of perinuclear mitochondrial in the hypoxia group were higher than those in the normal oxygen group,the differences were statistically significant (P＜0.05).Meanwhile,the significant difference was found in the ATP level between the two groups (P＜0.05).Conclusion The distribution of mitochondria in MEFs and energy synthesis are influenced by the hypoxic culture condition,which could be better for promoting cell growth compared with normal oxygen culture condition.关键词
低氧/小鼠胚胎成纤维细胞/线粒体分布/腺苷三磷酸Key words
hypoxia/mouse embryonic fibroblasts/mitochondrial distribution/adenosine triphosphate分类
医药卫生引用本文复制引用
王春,魏含清,裴轶劲..低氧培养对小鼠胚胎成纤维细胞生长和线粒体分布及功能的影响[J].重庆医学,2017,46(19):2599-2603,5.基金项目
国家自然科学基金项目(81173136) （81173136）
广东省科技计划项目(2013B022000003) （2013B022000003）
广东省基础与应用基础研究专项(广东省自然科学基金)(2015A030313524) （广东省自然科学基金）
广东省东莞市国际科技合作(含港澳台)项目(2015508102004). （含港澳台）
