重庆医学2017，Vol.46Issue(19)：2604-2607,2611,5.DOI:10.3969/j.issn.1671-8348.2017.19.004
重组质粒pcDNA3.1/C-sis对大鼠FHF的影响
The effect of the recombinant plasmid pcDNA3.1/C-sis on FHF of rats
摘要
Abstract
Objective To explore the effect of the recombinant plasmid pcDNA3.1/C-sis on fulminant hepatic failure (FHF) in rats.Methods 48 h after recombinant plasmid pcDNA3.1/C-sis being imported into rat liver by using the method of fluid mechanics,FHF in rats was induced by endotoxin (LPS)+D-galactosamine (D-GaIN).With fluorescence quantitative PCR and Western blotting,C-sis expression was tested.The apoptosis of rat liver was detected by using HE staining and measuring Caspase-3 activity.The expression changes of Bcl-2 and Bax were examined through using Western blotting.The mortality rate of rats was calculated during 24 h observation period.Resnlts Compared with the normal control group and FHF+ empty plasmid group,C-sis mRNA and protein expression levels were increased significantly in the FHF+C-sis plasmid group,there were statistically significant differences (P＜0.01).Compared with the normal control group,the apoptotic hepatocytes were increased in the FHF + Ringer's solution injection group and FHF+ empty plasmid group;compared with FHF+ empty plasmid group,the apoptotic hepatocytes in the FHF+C-sis plasmid group were decreased.Compared with the normal control group,Caspase-3 expression level was increased in the FHF+ Ringer's solution injection group (P＜0.01);compared with the FHF+ empty plasmid group,Caspase-3 expression level in the FHF+C-sis plasmid group was decreased (P＜0.05).Compared with the normal control group,Bcl-2 expression level was decreased significantly (P＜0.01),and Bax expression level was increased significantly (P＜0.01) in the FHF+Ringer's solution injection group;compared with the FHF+ empty plasmid group,the Bcl-2 expression level was increased (P＜0.05),and Bax expression was decreased (P＜0.05)in the FHF+C-sis plasmid group.During the 24 h observation period,all rats in the normal control group were alive;the mortality rates of the FHF+ Ringer's solution injection group and FHF+ empty plasmid group were 70.0％ and 80.0％ respectively,while that of the FHF+C-sis plasmid group was only 20.0％.Conclusion C-sis gene could inhibit FHF in rats induced by LPS+D-GalN.关键词
原癌基因蛋白质C-sis/重组质粒/大鼠/肝功能衰竭,急性Key words
proto-oncogene proteins C-sis/recombinant plasmid/rats/liver failure, acute分类
医药卫生引用本文复制引用
蔡鹏程,元文峰,丁浩..重组质粒pcDNA3.1/C-sis对大鼠FHF的影响[J].重庆医学,2017,46(19):2604-2607,2611,5.基金项目
国家自然科学基金资助项目(81300348) （81300348）
江西省青年科学基金资助项目(20151BAB215006) （20151BAB215006）
江西省教育厅科学技术研究项目(GJJ150259) （GJJ150259）
江西省卫生计生委科技计划项目(20165199). （20165199）
