重庆医学2017，Vol.46Issue(19)：2615-2617,3.DOI:10.3969/j.issn.1671-8348.2017.19.007
孕乳期补充维生素D对仔鼠生长发育及细胞免疫功能的影响
Effects of Vitamin D on growth and immune function in neonatal mice
摘要
Abstract
Objective To study the effects of Vitamin D (VitD) supplementation on growth and immune function in neonatal mice.Methods A total of 120 mice were divided into four groups (30 mice in each group) according to dose of VitD.The high-dose group,medium-dose group and low-dose group was given 3.44,1.72,0.86 IU VitD drops,respectively.The control group was not treated with VitD drops.Rat body weight,level of peripheral blood 25-(OH)D3 were observed.The cellular immune function (determined by using delayed hypersensitivity reaction experiment),humoral immune function (assessed by antibody producing cells counts and HC50 determination) and mononuclear-macrophage phagocytic function (assessed by mouse peritoneal macrophage phagocytosis of chicken red blood cells test and carbon clearance test) were detected.The flow cytometry assay was carried out to differentiate T lymphocyte subsets.Results With the increase of dose of VitD,levels of peripheral blood 25-(OH)D3 and calcium ion were gradually increased,there were statistically significant differences when compared with the control group(P＜0.05);the body weight and body length were gradually increased,while no statistically significant difference was found among the groups treated with VitD(P＞0.05).Compared to the control group,the toes swelling,phagocytic percentage,number of antibody producing cells,serum soluble HC50 in the high-dose group and medium-dose group were increased significantly,while carbon clearance test phagocytic index were decreased significantly (P＜0.05).With the increase of dose of VitD,the number of CD4+,CD8+ T lymphocytes and the CD4+/CD8+ ratio were gradually increased.Conclusion VitD could promote the growth and development of offspring mice,and enhance the immune function of the body.关键词
维生素D/免疫功能/小鼠Key words
Vitamin D/immune function/mice分类
医药卫生引用本文复制引用
黄利华,胡庆兰,李伟明,柳国胜,李宜花..孕乳期补充维生素D对仔鼠生长发育及细胞免疫功能的影响[J].重庆医学,2017,46(19):2615-2617,3.基金项目
清远市产业技术研究与开发资金项目(2013A014). （2013A014）
