重庆医学2017，Vol.46Issue(19)：2631-2634,4.DOI:10.3969/j.issn.1671-8348.2017.19.012
BBC3基因表达与肺鳞状细胞癌预后的相关性及其分子机制研究
Study on the relationship between BBC3 expression and prognosis of patients with squamous cell lung neoplasms and its mechanism
摘要
Abstract
Objective To explore the correlation between BCL2 binding component 3 (BBC3) expression and postoperative survival time of lung squamous cell carcinoma (LUSC),and investigate its molecular mechanism.Methods The expression of BBC3 in 39 patients was detected by using qRT-PCR assay.Meanwhile,clinical data of all patients were collected by follow-up visit.Then,the survival analysis was performed by using Kaplan-Meier and log rank tests.Moreover,multiple factors analysis was performed using COX proportional hazard model.At last,BBC3 was over-expressed in NCI-H226 cell lines,then detected the effects of BBC3 expression on cell proliferation and apoptosis by using MTT assay and flow cytometry.Results BBC3 expression were significantly correlated with the tumor metastasis (r=0.556,P=0.023),tumor size (r=0.532,P =0.042),T staging (r =0.551,P=0.021) and TNM staging (r=0.524,P=0.047).Meanwhile,the results of Kaplan-Meier and log rank tests found that BBC3 expression was significantly correlated with survival time of patients with LUSC,and the length of survival time in patients with high BBC3 expression was longer than that in patients with low BBC3 expression (x2 =7.542,P=0.006).The COX proportional hazard model indicated that tumor metastasis,T staging,TNM staging and BBC3 expression were independent factors which significantly affected survival time.Moreover,the proportion of apoptotic cells in the recombinant plasmid BBCs group was higher than that in the empty plasmid group and control group (P＜0.05).Conclusion BBC3 expression could suppress the proliferation of tumor cells and promote apoptosis,and are significantly correlated with survival time of patients,so which may be assistant biomarkers for prognosis of LUSC.关键词
BBC3/肺/肿瘤,鳞状细胞/预后/细胞增殖/细胞凋亡/生物学标记Key words
BBC3/lung/neoplasms, squamous cell/prognosis/cell proliferation/apoptosis/biological markers分类
医药卫生引用本文复制引用
李超,沈学远,胡煜琳..BBC3基因表达与肺鳞状细胞癌预后的相关性及其分子机制研究[J].重庆医学,2017,46(19):2631-2634,4.基金项目
重庆市科委一般项目(cstc2016jcyjA0343) （cstc2016jcyjA0343）
重庆市卫生局科研面上项目(2015MSXM058) （2015MSXM058）
重庆市永川区自然科学基金(Ycstc,2015nc5004) （Ycstc,2015nc5004）
重庆医科大学附属永川医院院内课题(YJZQN201529,YJSCI201504). （YJZQN201529,YJSCI201504）
