动物营养学报2017，Vol.29Issue(7)：2407-2415,9.DOI:10.3969/j.issn.1006-267x.2017.07.024
饲粮中短期内单独及混合添加高水平燕麦β-葡聚糖和微晶纤维素对小鼠生长性能、器官指数和粪便细菌群落结构的影响
Short-Term Adding High-Level Oat β-Glucan, Microcrystalline Cellulose and Their Mixture in Diets Affects Growth Performance, Organ Indexes and Fecal Bacterial Community Structure of Mice
摘要
Abstract
This experiment was conducted to investigate the effects of adding high-level oat β-glucan, microcrystalline cellulose as well as their mixture in diets on the growth performance, organ indexes and fecal bacterial community structure of mice with a short-term feeding experiment.Thirty-six healthy BALB/c mice with the body weight of (17.95±0.95) g were selected and randomly allocated to four groups according to body weight, and each group had nine mice.Mice in control group (CON group) were fed a diet without oat β-glucan and microcrystalline cellulose, while mice in the other three groups were fed diets with 28% oat β-glucan (G group), 20% microcrystalline cellulose (M group), and 14% oat β-glucan+10% microcrystalline cellulose (GM group), respectively.Each animal was raised in a single cage, and the experiment lasted for 21 days.The results showed as follows: 1) during the whole experimental period, the average daily gain (ADG) of mice showed no significant difference among groups (P>0.05), but the average daily feed intake (ADFI) of mice showed significantly different among groups (P<0.05), and the trend was presented as G group<M group<GM group<CON group.2) The spleen index of mice in the four groups showed no significant difference (P>0.05).The epididymal fat index of mice showed no significant difference between CON group and the three fiber supplemented groups (G, M and GM groups) (P>0.05), but it showed significantly lower in G and M groups than GM group (P<0.05).3) Bacterial Shannon-Wiener index of feces of mice in G group was significantly lower than that in CON group (P<0.05).According the cluster analysis of polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) profile, fecal bacterial community structure of mice had obvious difference in among groups, fecal samples from each group could be clustered into separate clade on experimental day 13 and 17.In conclusion, short-term adding high-level oat β-glucan, microcrystalline cellulose or their mixture in diets can decrease the ADFI of mice, bur with no effect on the ADG and spleen index.The supplementation of oat β-glucan and microcrystalline cellulose mixture stimulates the epididymal fat deposition rather than the supplementation of single-type fiber.The high-level oat β-glucan can decrease the bacterial diversity in the hindgut of mice.Both of the oat β-glucan and microcrystalline cellulose can alter the fecal microflora of mice, indicating that there may be different core bacterial groups specifically utilizing the two types of dietary fibers.关键词
燕麦β-葡聚糖/微晶纤维素/BABL/c小鼠/生长性能/器官指数/粪便微生物区系Key words
oat β-glucan/microcrystalline cellulose/BABL/c mice/growth performance/organ indexes/fecal microflora分类
农业科技引用本文复制引用
张玲,罗玉衡,陈代文,余冰,何军,虞洁,罗钧秋,毛湘冰,黄志清,郑萍..饲粮中短期内单独及混合添加高水平燕麦β-葡聚糖和微晶纤维素对小鼠生长性能、器官指数和粪便细菌群落结构的影响[J].动物营养学报,2017,29(7):2407-2415,9.基金项目
国家自然科学基金项目(31301987) （31301987）
