动物营养学报2017，Vol.29Issue(7)：2482-2491,10.DOI:10.3969/j.issn.1006-267x.2017.07.033
低淀粉饲粮条件下不同瘤胃降解淀粉水平对体外瘤胃发酵的影响
Effects of Different Ruminally Degradable Starch Levels in Low Starch Diet on in Vitro Ruminal Fermentation
摘要
Abstract
This trial was performed to examine the effects of different ruminally degradable starch levels in the diet with low corn-based starch on in vitro ruminal fermentation.Three Holstein cows fitted with permanent rumen fistulas were used as the donor of rumen fluid.Diets with different ruminally degradable starch levels were used as substrates, and the changes of gas production and ruminal fermentation parameters during 48 h incubation were determined using in vitro gas production method, and the ruminal microflora change during 24 h incubation was also determined.The results showed as follows: 1) with the ruminally degradable starch level increasing, gas production (GP), potential gas production (a+b) and gas production rate constant for "gas production from the slowly soluble fraction (b)" (c) were linearly increased (P<0.05), the gas production from the immediately soluble fraction (a) was linearly decreased (P<0.05), and the dry matter digestibility was linearly increased after 48 h in vitro incubation (P<0.05).2) With the ruminally degradable starch level increasing, fermentation fluid microprotein (MCP), acetate, propionate, butyrate and total volatile fatty acids (TVFA) concentrations were linearly increased after 48 h in vitro incubation (P<0.05), but the pH and ammoni anitrogen concentration were not significantly changed (P>0.05).3) With the ruminally degradable starch level increasing, the relative counts of R.albus and R.amylophilus in fermentation fluid after 24 h in vitro incubation were linearly increased (P<0.05), while the relative counts of R.flavefacien, F.succinogenes, B.fibrisolvens, S.bovis and S.amylolytica had no significant changes (P>0.05).It is concluded that incraesing ruminally degradable starch level can improve ruminal fermentation under the condition of low starch diet.关键词
瘤胃降解淀粉/产气量/瘤胃发酵/微生物区系Key words
ruminally degradable starch/gas production/rumen fermentation/microflora分类
农业科技引用本文复制引用
雒国彬,王利军,刘岩,张广宁,孙凯晶,王馨影,张永根..低淀粉饲粮条件下不同瘤胃降解淀粉水平对体外瘤胃发酵的影响[J].动物营养学报,2017,29(7):2482-2491,10.基金项目
国家奶牛产业技术体系项目(CARS-37) （CARS-37）
