动物营养学报2017，Vol.29Issue(7)：2535-2540,6.DOI:10.3969/j.issn.1006-267x.2017.07.038
硫酸化银耳多糖和党参多糖对鸡T淋巴细胞增殖及白细胞介素-2 mRNA表达水平的影响
Effect of Sulfated Polysaccharidses from Tremella and Condonpsis pilosula on T Lymphocyte Proliferation and mRNA Expression Level of Interleukin-2 of Broilers
摘要
Abstract
In order to study the mechanism of sulfated polysaccharidses from Tremella (sTPS70c) and Condonpsis pilosula (sCPPS50c) on immunological enhancement activity, the effects of sTPS70c and sCPPS50c on T lymphocyte proliferation and the mRNA expression level of interleukin-2 (IL-2) were determined by methyl thiazolyl tetrazolium (MTT) assay and real-time fluorescent quantitative PCR assay while taking the unmodified polysaccharidses from Tremella (TPStp) as control.The results showed that in single adding into peripheral lymphocyte, sTPS70c and sCPPS50c almost at all concentrations and TPStp only at 3.125 μg/mL could significantly stimulate T lymphocyte proliferation (P<0.05).In simultaneous adding into peripheral lymphocyte with plant haemagglutinin P (PHA-P), sCPPS50c at 0.391 to 1.563 μg/mL significantly stimulated T lymphocyte proliferation (P<0.05).sTPS70c and sCPPS50c also could significantly promote mRNA expression level of IL-2 in T lymphocyte at 1.563 μg/mL (P<0.05), and the promoting effects of sTPS70c were significantly better than TPStp (P<0.05), especially at 1.563 μg/mL, the effect of sTPS70c was the strongest.These results indicate that sulfated modification can enhance the lymphocyte proliferation and mRNA expression of IL-2, and sTPS70c presents more stronger action, which is related to the degree of substitution at a centain extent.关键词
硫酸化银耳多糖/硫酸化党参多糖/淋巴细胞增殖/白细胞介素-2Key words
sulfated Tremella polysaccharide/sulfated Codonopsis pilosula polysaccharide/lymphocyte proliferation/interleukin-2分类
农业科技引用本文复制引用
朱广双,胡元亮,曹侃,王本忠..硫酸化银耳多糖和党参多糖对鸡T淋巴细胞增殖及白细胞介素-2 mRNA表达水平的影响[J].动物营养学报,2017,29(7):2535-2540,6.基金项目
安徽省教育厅自然科学重点项目(KJ2017A564) （KJ2017A564）
安徽省教育厅自然科学重点项目(KJ2015A401) （KJ2015A401）
校级自然科学研究项目"应用复方中草药防治猪场常见呼吸道疾病的研究"(wzyzr201619) （wzyzr201619）
校级"教学质量与教学改革工程"项目(2016) （2016）
