动物营养学报2017，Vol.29Issue(7)：2587-2596,10.DOI:10.3969/j.issn.1006-267x.2017.07.044
海带渣添加比例及其酶解产物对凡纳滨对虾生长、消化和非特异性免疫力的影响
Effects of Kelp Meal Adding Proportion and Its Enzymatic Hydrolysates on Growth, Digestion and Non-Specific Immunity of Litopenaeus vannamei
摘要
Abstract
This study focused on evaluating the effects of kelp meal adding proportion and enzymatic hydrolysates on growth, digestion and non-specific immunity of Litopenaeus vannamei.There were two experiments in this study: the optimal adding proportion experiment of kelp meal and the effect experiment of kelp meal enzymatic hydrolysates.The optimal adding proportion experiment of kelp meal (experiment one): according to a single factor experimental design, six diets were formulated to contain 0, 0.5%, 1.0%, 2.0%, 3.0% and 5.0% kelp meal (named as groups D1 to D6, respectively), respectively, fed the shrimps [initial body weight was (1.00±0.10) g] for 49 days, and then the growth performance indices, activities of digestive enzymes in stomach and non-specific immune enzymes in serum of Litopenaeus vannamei were measured.There were 3 replicates per group with 35 shrimps per replicate.The effect experiment of kelp meal enzymatic hydrolysates (experiment two): a diet with 3.0% kelp meal was kelp meal control group (group A) that based on the experiment one had the optimal adding proportion, and then 3.0% kelp meal after β-glucanase (group B) and protease treatment (group C) were used to replace an equal amount of kelp meal, respectively.The Litopenaeus vannamei with the initial body weight of (1.00±0.10) g were fed those diets for 56-day, the growth performance indices, activities of digestive enzymes in stomach and non-specific immune enzymes in serum of Litopenaeus vannamei were measured.There were 3 replicates per group with 35 shrimps per replicate.The results of experiment one showed as follows: the weight gain rate (WGR) and specific growth rate (SGR) in groups D4 and D5 were significantly higher than those in control group (P<0.05).In addition, the feed conversion ratio (FCR) in D5 group was significantly lower than that in control group (P<0.05).With the kelp meal adding proportion increasing, the activities of pepsin (PP) in stomach, and superoxide dismutase (SOD) and phenoloxidase (PO) in serum were firstly increased and then decreased.The activities of those three kinds of enzymes reached the maximum in D5 group, and the activities of serum PO and SOD in D5 group were significantly higher than those in control group (P<0.05).The results of experiment two showed as follows: compared with kelp meal treated without enzyme, kelp meal treated by β-glucanase had significant influence on improving WGR, SGR and reducing FCR (P<0.05), and kelp meal treated by protease can significantly increase survival rate (SR) (P<0.05).The cellulase activity in stomach of groups B and C was significantly decreased compared with group A (P<0.05).The activities of acid phosphatase (ACP) and PO in serum of groups B and C and the serum SOD activity of group C were significantly higher than those of group A (P<0.05).It is suggested that the optimal adding proportion of kelp meal in the diet of Litopenaeus vannamei is 3.0%.Kelp meal treated by β-glucanase can improve the growth performance and non-specific immunity of Litopenaeus vannamei, and treated by protease can improve non-specific immunity, but has no enhancement on growth of Litopenaeus vannamei.关键词
海带渣/酶解产物/南美白对虾/生长性能/消化酶/非特异性免疫酶Key words
kelp meal/enzymatic hydrolysates/Litopenaeus vannamei/growth performance/digestive enzymes/non-specific immune enzymes分类
农业科技引用本文复制引用
彭素晓,常志强,马骊,李健..海带渣添加比例及其酶解产物对凡纳滨对虾生长、消化和非特异性免疫力的影响[J].动物营养学报,2017,29(7):2587-2596,10.基金项目
国家虾现代产业技术体系(CARS-47) （CARS-47）
青岛海洋科学与技术国家实验室鳌山科技创新计划子任务"海水池塘健康养殖技术研发"(2015ASKJ02-03) （2015ASKJ02-03）
