南方医科大学学报2017，Vol.37Issue(7)：861-865,5.DOI:10.3969/j.issn.1673-4254.2017.07.01
医院获得性感染中出现携带SCCmecⅣ/Ⅴ的耐甲氧西林金黄色葡萄球菌
Emergence of methicillin-resistant Staphylococcus aureus SCCmec type IV/V epidemic clones in a large teaching hospital in China
摘要
Abstract
Objective To investigate the staphylococcal cassette chromosome mec (SCCmec) genotype and molecular epidemiological characteristics of healthcare-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) in a large teaching hospital in China. Methods From January 2012 to December 2012, a total of 71 nonduplicate HA-MRSA were collected in a teaching hospital in Changsha, China. SCCmec types were determined by multiplex PCR, and Panton-Valentine leukocidin (PVL) gene was detected by PCR. The homology among the tested isolates was determined using pulsed-field gel electrophoresis (PFGE). Results Of the 71 HA-MRSA isolates, 49 (69.0%) carried SCCmec Ⅲ, 10 (14.1%) carried SCCmec IV, 3 (4.2%) carried SCCmec V and 3 (4.2%) carried SCCmec II;the remaining 6 isolates were not typeable by PCR. Compared with patients having SCCmec Ⅰ/Ⅱ/Ⅲ MRSA infections, those with SCCmec IV/V MRSA infections had a significantly younger age and a similar duration of hospital stay before the first MRSA-positive culture and total hospital stay. PVL genes were strongly associated with SCCmec type IV/V MRSA infections. HA-SCCmec IV/V MRSA strains showed a greater susceptibility to rifampicin, gentamicin, levofloxacin, ciprofloxacin, and tetracycline than HA-SCCmec Ⅰ/Ⅱ/Ⅲ MRSA strains. The 13 HA-SCCmec IV/V MRSA isolates formed one large group at the 55%similarity level. Three PFGE clusters with a similarity index of 85%or more were identified, and unique PFGE profiles were observed in 4 isolates. Conclusions This is the first report of HA-MRSA isolates carrying SCCmec V in Chinese hospitals. SCCmec types IV and V MRSA clones have emerged in Chinese hospitals, which urges more rigorous surveillance of their spread in healthcare facilities in China.关键词
耐甲氧西林金黄色葡萄球菌/医院感染/葡萄球菌染色体mec基因盒/PVL基因/脉冲场凝胶电泳Key words
methicillin-resistant Staphylococcus aureus/nosocomial infection/SCCmec/Panton-Valentine leukocidin genes/pulsed field gel electrophoresis引用本文复制引用
钟一鸣,袁瑞,丁健生,杨芳,刘文恩..医院获得性感染中出现携带SCCmecⅣ/Ⅴ的耐甲氧西林金黄色葡萄球菌[J].南方医科大学学报,2017,37(7):861-865,5.基金项目
国家自然科学基金(81672066) （81672066）
湖南省发改委基金项目([2014]658) Supported by National Natural Science Foundation of China (81672066) and a grant from the Development and Reform Commission of Hunan Province ([2014]658). （[2014]658）
