南方医科大学学报2017，Vol.37Issue(7)：869-874,6.DOI:10.3969/j.issn.1673-4254.2017.07.03
S100A4:潜在抑制膀胱癌干细胞的靶分子
S100A4, a potential therapeutic target on bladder cancer stem cells
摘要
Abstract
Objective To observe the effect of S100A4 gene silencing mediated by small interfering RNA (siRNA) on the proliferation of bladder cancer stem cells (CSCs) and their capacity of xenograft tumor formation. Methods MB49 bladder cancer stem cells (MCSCs) were isolated and identified. The differentially expressed protein S100A4 was identified in MCSCs using isobaric tags for relative and absolute quantitation technology (iTRAQ). A siRNA targeting S100A4 was constructed and transfected into MCSCs, and its inhibitory effects on S100A4 expression in MCSCs were assessed with Western blotting and qPCR. The effects of siRNA-mediated S100A4 silencing on the proliferation and xenograft tumor formation ability of MCSCs were observed. Results Among the 65 differentially expressed proteins identified by iTRAQ combined with LC/MS/MS, S100A4 protein showed the most distinct differential expression in MCSCs. Transfection of MCSCs with S100A siRNA significantly inhibited the expressions of S100A4 at both mRNA and protein levels, caused obvious suppression of the cell proliferation, and attenuated the xenograft tumor formation ability of the cells in nude mice. Conclusion S100A4 in MCSCs is associated with the recurrence and metastasis of bladder cancer. S100A4 may serve as a potential therapeutic target for eliminating bladder CSCs.关键词
膀胱癌干细胞/S100A4/增殖/成瘤Key words
bladder cancer stem cells/S100A4/proliferation/xenograft tumor formation ability引用本文复制引用
王春燕,聂庆文,周璇,黄大雄,肖炜强,朱永通..S100A4:潜在抑制膀胱癌干细胞的靶分子[J].南方医科大学学报,2017,37(7):869-874,6.基金项目
广东省自然科学基金(2016A030310403) （2016A030310403）
广东省医学科学技术研究基金(A2016092) （A2016092）
