南方医科大学学报2017，Vol.37Issue(7)：895-901,7.DOI:10.3969/j.issn.1673-4254.2017.07.07
血管紧张素-(1-7)通过抑制ClC-3通道减轻高糖引起的心肌细胞损伤
Angiotensin-(1-7) protects cardiac myocytes against high glucose-induced injury by inhibiting ClC-3 chloride channels
摘要
Abstract
Objective To explore whether angiotensin-(1-7) [Ang-(1-7)] protects cardiac myocytes against high glucose (HG)-induced injury by inhibiting ClC-3 chloride channels. Method H9c2 cardiac cells were exposed to 35 mmol/L glucose for 24 h to establish a cell injury model. The cells were treated with Ang-(1-7) or the inhibitor of chloride channel (NPPB) in the presence of HG for 24 h to observe the changes in HG-induced cell injury. Cell counter kit 8 (CCK-8) assay was used to test the cell viability, and the morphological changes of the apoptotic cells were detected using Hoechst 33258 staining and fluorescent microscopy. The intracellular level of reactive oxygen species (ROS) was examined by DCFH-DA staining, SOD activity in the culture medium was measured using commercial kits, and the mitochondrial membrane potential (MMP) of the cells was tested with rodamine 123 staining. The expression level of cardiac ClC-3 chloride channels was detected with Western blotting. Results Exposure of H9c2 cardiac cells to 35 mmol/L glucose for 24 h markedly enhanced the expressions of cardiac ClC-3 channel protein (P<0.01). Co-treatment of the cells with 1 μmol/L Ang-(1-7) and HG for 24 h significantly attenuated HG-induced upregulation of ClC-3 channel protein expression (P<0.01). Co-treatment of the cells exposed to HG with 1 μmol/L Ang-(1-7) or 100 μmol/L NPPB for 24 h obviously ameliorated HG-induced injuries as shown by increased cell viability, enhanced SOD activity, decreased number of apoptotic cells, and reduced intracellular ROS generation and loss of MMP (P<0.01). Conclusion ClC-3 channels are involved in HG-induced injury in cardiac cells. Ang-(1-7) protects cardiac cells against HG-induced injury by inhibiting ClC-3 channels.关键词
血管紧张素-(1-7)/ClC-3通道/高糖/心肌细胞/损伤Key words
angiotensin-(1-7)/ClC-3 chloride channels/high glucose/cardiac cells/cell injury引用本文复制引用
蔡少艾,陈景福,陈美姬,林健聪,冯鉴强,林凯,智喜梅,张伟杰,吴文..血管紧张素-(1-7)通过抑制ClC-3通道减轻高糖引起的心肌细胞损伤[J].南方医科大学学报,2017,37(7):895-901,7.基金项目
广东省自然科学基金(2016A030313570) （2016A030313570）
广州市黄埔区科技计划项目(201544-01) （201544-01）
