南方医科大学学报2017，Vol.37Issue(7)：907-913,7.DOI:10.3969/j.issn.1673-4254.2017.07.09
乳酸抑制精液来源淀粉样纤维的形成
Lactic acid inhibits the formation of semen-derived amyloid fibrils
摘要
Abstract
Objective To investigate the inhibitory effect of lactic acid on semen-derived amyloid (SEVI) fibril formation. Methods PAP248-286 (2 mg/mL) was incubated with 4.0, 2.0, 1.0, 0.5, 0.25, and 0.125 mg/mL of lactic acid. After incubation for different times, aliquots were drawn from each sample for Thioflavin T (ThT) and Congo red staining to monitor semen-derived amyloid fibril formation. The β sheet structure formation of PAP248-286 was measured by circular dichroism spectrum, and the morphology of amyloid fibrils incubated with or without lactic acid was observed with transmission electron microscopy (TEM). The enhancing effect of amyloid fibril incubated with lactic acid at different time points was determined using virus infection assay. PAP248-286 (2 mg/mL) was incubated with dilutions of vaginal secretion from healthy women, and amyloid fibril formation was detected with ThT and Congo red staining. Results Lactic acid inhibited SEVI fibril formation in a dose-dependent manner in vitro. Lactic acid at 0.5 mg/mL completely inhibited 2 mg/mL SEVI fibril formation within 48 h. After incubation for 48 h, lactic acid at 1 mg/mL inhibited the formation ofβ-sheet structure of SEVI (2 mg/mL) and completely inhibited 2 mg/mL PAP248-286 aggregation as observed with TEM. In the presence of lactic acid, PAP248-286 lost the ability to enhance virus infection. Vaginal secretion inhibited SEVI fibril formation in a dose-dependent manner, and virtually no SEVI fibril occurred after incubation of 2 mg/mL PAP248-286 with 67%vaginal secretion. Conclusion Lactic acid inhibits SEVI fibril formation in vitro.关键词
乳酸/淀粉样纤维/人类免疫缺陷病毒/性传播/阴道分泌液Key words
lactic acid/amyloid fibril/HIV/sexual transmission/vaginal secretion引用本文复制引用
李锦清,宋亚丽,寻添荣,谭穗懿,刘叔文..乳酸抑制精液来源淀粉样纤维的形成[J].南方医科大学学报,2017,37(7):907-913,7.基金项目
国家自然科学基金(31370781) （31370781）
广州市珠江科技新星(201610010132) Supported by National Natural Science Foundation of China (31370781). （201610010132）
