南方医科大学学报2017，Vol.37Issue(7)：914-918,5.DOI:10.3969/j.issn.1673-4254.2017.07.10
经股动脉注射途径建立前列腺癌和乳腺癌骨转移模型
Establishment of mouse model of bone metastasis of prostate cancer and breast cancer via femoral artery injection of tumor cells
摘要
Abstract
Objective To explore an efficient and reliable method for establishing an animal model of bone metastasis of tumors. Methods Male C57BL/6J mice were randomized into 4 equal groups to receive injections of normal saline or prostate cancer RM-1 cell suspension via the femoral artery or the external iliac artery, and breast cancer 4T1-luc cells were injected in 15 female BALB/c mice via the femoral artery. The operation time, postoperative survival rate, and the tumor formation rates in the mice with two different injection methods were compared. The tumor metastasis in the mice was evaluated with in vivo imaging. Results The mean time for hemostasis time was 2.53 ± 1.75 min in mice receiving tumor cell injection via the femoral artery, significantly shorter than that in mice with injections via the external iliac artery (4.70 ± 1.63 min; P<0.05); the mean operation time was 14.67±2.16 min and 22.47±3.50 min in the two groups, respectively (P<0.05). At 21 days after the operation, the survival rate was 93.3% in femoral artery injection group, significantly higher than that in external iliac artery injection group (66.7%; P<0.05). The tumor metastasis rate was 100% in both groups. Conclusion Injection of the tumor cells via the femoral artery is more suitable for establishing mouse models of bone metastasis of cancers.关键词
前列腺癌/乳腺癌/骨转移/动物模型Key words
prostate cancer/breast cancer/bone metastasis/animal models引用本文复制引用
殷昭阳,齐思勇,成祥,郭靓,陈泓宇,施明..经股动脉注射途径建立前列腺癌和乳腺癌骨转移模型[J].南方医科大学学报,2017,37(7):914-918,5.基金项目
国家自然科学基金(81572845,81402562) Supported by National Natural Science Foundation of China(81572845, 81402562). （81572845,81402562）
